Ketchup: Methods of Manufacture; Microscopic ExaminationBitting, K. G. (Katherine Golden)
Science
Ketchup: Methods of Manufacture; Microscopic Examination
Bitting, K. G. (Katherine Golden)
Ketchup
The examination for mold is made with the ×6 ocular and 16 mm objective,
giving a magnification of approximately 90 times. About 50 fields are
supposed to be examined and the result expressed in terms of the per
cent in which mold was found. It is stated that it is believed that
manufacturers can conduct their operations so that mold will not be
present in more than 25 per cent of the fields. There are, therefore,
three units in which to express the results: bacteria in cubic
centimeters, yeasts and spores in one-sixtieth of a cubic millimeter,
and molds in percentage of microscopic fields.
Aside from the errors which may occur in the manipulation of the purely
mechanical part of the technique, there are other considerations which
affect the accuracy of the results. First, the differentiation between
organisms and tissues is not considered possible by most pathologists
and bacteriologists without differential staining. Even in such simple
examinations as those for diphtheria and tuberculosis, a stain is
required. In foods the particles of the plant tissue and the organisms
are not so different that they can be clearly separated without using
similar technique. It is possible to make some separation, but not with
accuracy. Threads of protoplasm may be mistaken for bacilli; the
granular contents of a cell for cocci, yeasts, or spores; bits of cell
wall for hyphae under the magnifications given, and the results obtained
be high or low, depending upon the personal ability of the operator.
Each error magnified by the enormous factor used in calculating the
final result naturally gives figures which may be far above or below the
truth. Those who have had special training in plant structure and
bacteriology are likely to give the higher figures, while those who have
had these subjects as incidentals in a scientific course are apt to give
much lower ones.
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