Ketchup: Methods of Manufacture; Microscopic ExaminationBitting, K. G. (Katherine Golden)
Science
Ketchup: Methods of Manufacture; Microscopic Examination
Bitting, K. G. (Katherine Golden)
Ketchup
Second. The standard is set for what organisms shall be counted and
those which need not be. It is said that micrococci need not be counted
because of the difficulty in distinguishing them from “particles of
clay, etc.,” and not upon their power to produce decomposition. When an
organism is a coccus and when rod shaped is not easily settled, even
with the aid of pure cultures and high power objectives. More than one
organism has found a home first in one group and then in the other, and
differentiation with the low power obtained by an 8 mm objective is
impossible. There are always present some very large rods, but there may
be more very short ones which may not be counted, and there is nearly
always a diplococcus present, which, with the magnification used, is
difficult to differentiate from a rod. There are four forms associated
with rot and tomato diseases which have been carefully studied—all rods,
but very small ones. Ps. fluorescence, 0.68×1.17-1.86; Ps. michiganense,
0.35-0.4×0.8-1.0; B. carotovorus, 0.7-1.0×1.5-5; and B. solanacearum,
0.5×1.5. Bacillus subtilis, .7×2-8 and some lactic acid forming
varieties are always present. It is clearly a matter of judgment on the
part of the examiner as to which organisms he will count and which he
will not attempt to count. A personal equation is thus introduced which
nullifies the possibilities of scientific accuracy.
The yeasts and spores are counted together. They can not be separated
under the microscope, neither can they be differentiated from contracted
protozoa which may be present in large numbers. In counting these, it is
not always possible to distinguish the smaller yeast cells and smaller
spores from the refractive bodies which are formed in some mold hyphae
when these are impoverished, and which are liberated if thorough shaking
of the sample be done. The yeasts found in pulp and ketchup are more
likely to be “wild yeasts” and these are, as a general thing, smaller
than the cultivated, sporulate more readily, and have more highly
refractive spores. Then, some of the so-called molds found form minute
conidia and when these and the yeasts are mixed with the detritus of the
tomato and the mass subjected to heat, with the consequent changes, the
accuracy of the count becomes a somewhat problematical matter. A careful
examination of the kind and condition of the hyphae present might assist
materially in making some distinction.
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