Natural History of the Ornate Box Turtle, Terrapene ornata ornata AgassizLegler, John M.
Science
Natural History of the Ornate Box Turtle, Terrapene ornata ornata Agassiz
Legler, John M.
Ornate box turtle
Testes of mature individuals are pale yellow and slightly oblong. The
epididymis is ordinarily dark brown or black and contrasts sharply
with the color of the testes. Size of testes was expressed as the
average length (greatest diameter) of both testes. Testes are smallest
in April, immediately after emergence from hibernation, and largest in
early September (Pl. 20, Figs. 3-4). They are nearly spherical when of
maximum size; increase in bulk, therefore, is relatively greater than
the increase in size shown in Figure 3. They increase in size from
April until early June, recede during most of June, and again increase
in size in July and August. They remain large from early September
until hibernation is begun, becoming only slightly smaller in late
September and October.
Increase in size following emergence from hibernation may be due in
part to proliferation of the sustentacular cytoplasm. Decrease in size
in early June is correlated with the end of the period of most active
mating; maximal size is coincident with the peak of the spermatogenic
cycle in early September.
[Illustration: FIG. 3. Seasonal fluctuations in size (average
greatest diameter) of testes in _T. o. ornata_ as determined
by examination of 40 specimens from eastern Kansas.]
Spermatogenesis (refer to Pl. 19, Figs. 1-5) begins in early May when
a few spermatogonia appear in the seminiferous tubules. The
histological appearance of testes preserved in April and May is much
the same. Nuclei of Sertoli cells, which outnumber the spermatogonia,
are evident at the periphery of the tubules and the clear cytoplasm of
the cells extends into and nearly fills the lumina. The few darkly
stained spermatids that are present in April are cells that probably
were produced in the previous summer. Sperm are present in small
groups within the sustentacular cytoplasm, but ordinarily are absent
in the lumina.
Primary spermatocytes appear in the tubules from mid-May to early
June. By mid-May there are practically no sperm at any place in the
tubules. The sustentacular cytoplasm has a less compact arrangement in
late May than in April.
Spermatogenesis is well under way by mid-June; at this time, two or
three distinct layers of primary and secondary spermatocytes are
present and these cells outnumber the Sertoli cells. The lumina are
filled with cellular detritus and are no longer bordered by a clear
ring of sustentacular cytoplasm. No sperm are present.
Spermatids appear in late June and a few of them undergo metamorphosis
in early July; by mid-July, spermatids and secondary spermatocytes are
the dominant cells in the seminiferous tubules, although
spermatogonia are still active.
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