On adipocire, and its formationWetherill, Charles Mayer
Science
On adipocire, and its formation
Wetherill, Charles Mayer
Adipocere; Fatty acids; Postmortem changes
4^o. Added an equal weight of alcohol and melted; pressed after two
days. The liquid by this time, was light yellowish; the fatty crystals
in white flakes or scales; the smaller ones transparent under the
microscope, and polarizable. A portion of the fat was melted, and
observed cooling under the microscope with polarized light; as the
solidification approached, a beautiful play of prismatic colours took
place, and the drop shot into crystal interlaced lamellæ. A drop
melted with alcohol, and let cool, gave the peculiar dendritic curved
appearance of margaric acid.
5^o. The fat by this time weighed 300 grammes; it was melted with an
equal weight of alcohol, and pressed the following day. Residue, 253
grammes.
6^o. This was melted with 250 alcohol; the liquid from the press was
very little less coloured than the last; the residue weighed 227
grammes, and was brilliant white, with a tinge of yellow; the fracture
showed large crystals, and could not be distinguished from the product
of the stearic candle factories. When melted, it cooled with raised,
uneven surface, and was completely soluble in ether. When the ethereal
solution was suffered to separate spontaneously, the first fat which
made its appearance melted at 60°, solidified at 55°, and the fat
extracted from the rest of the ether gave exactly the same points.
The following are the melting points yielded by the fatty residues of
the foregoing alcoholic crystallizations:
Fat melts solidifies
2^o 58° 53°
3^o 58° 53° a 52°
4^o 58° 53° a 52°
5^o 58° a 58.5° 53°
6^o 60° 55° a 54°
The examination of the liquids separated from the above
crystallizations, was now taken up. Their colour was from a very deep
reddish brown (No. 1^o) down to light yellow, and nearly colourless
(No. 6^o.) In 1^o, 2^o, and 5^o, crystals had deposited by standing,
and as 2^o was not corked like the rest, the deposit here was abundant;
it was re-melted with addition of as much alcohol as had evaporated,
and was suffered to stand for several days longer, when a drusy
crystalline deposit made its appearance. The following are the melting
points for these two precipitates:
Precipitate in 1^o melts 62°-63° solidifies 44°5-40°
Precipitate in 5^o melts 51°-56° solidifies 44°5-43°
The fat deposited in 2^o melted at 58°, and solidified at 52°, and
the fat separated from the _liquid_ of this bottle, melted at 59°5,
solidified at 53°, but continued translucent down to 33°. After the
above melting points, 1^o and 5^o were observed, the same fat was
raised slowly to the melting points, and then kept for a considerable
time in the thermostat, at 100°, the points were again determined, and
found to be the same.
The liquids separated from the fats 1^o-6^o, gave the following results:
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