45. Eighth oral lobe was removed and pulsations stopped. The next day the animal was in good condition. The pulsations counted in the evening were 12, 14, 14, 11, per minute. The rhythm was not regular; there was a tendency to groups of twos, threes, or more, but no prolonged intervals of rest were observed. When placed into fresh sea-water, the pulsations were fourteen to the half-minute or twenty-six to the minute; seventeen to the half-minute, and thirty-three to the minute were also counted. This specimen gave spontaneous contractions during two weeks, after which it was thrown out, the aboral end being eaten through and little or no regeneration having taken place. 46. Two more were operated upon: A. Its rhythm was 18, 14, 17. Its entire margin was cut off. The separate pieces of the margin pulsated, 6, 7, 4, 6, 7, 9. The animal seemed paralyzed by the operation; it responded by a contraction now and then to stimulation but gave no spontaneous pulsations. B. Its rhythm was 17, 15, 12, 12. All its _oral arms_ were removed. Its rhythm was only raised to seventeen and not perfect. In twenty-five minutes it had fallen to eleven, in four hours to ten pulsations [per minute]. May 22nd. A and B are living as also the pieces of the _margin_ of A; all are giving spontaneous pulsations now and then at comparatively long intervals--even A, with its margin removed. May 26th. Everything is still living. The one with the margin cut (A) counted sixteen and nineteen pulsations per minute, though this was not kept up all the time. June 2nd. A and B and pieces are still living and contracting spontaneously. It is now two weeks, and they were thrown out eaten through at the aboral end with little or no regeneration. 47. The margin was cut off another one (C) and it was then paralyzed. The margin contracted vigorously by itself. The margin was next split, but a connection of about one-half an inch wide was left between the two rings. Over this bridge the contractions passed from the outer and inner ring. The inner ring did not originate any contractions. Both rings were then cut near their connecting bridge of tissue and the larger ring with the marginal bodies was split longitudinally so as to separate the exumbral from the subumbral portion. It was found that the contractions started only from the subumbral portion while the exumbral portion did not contract at all. June 5th. Five of the eight small pieces of C were not seen to contract either to-day or yesterday. A slow rotary motion was observed in some of the pieces suggesting ciliation, but no cilia or currents pointing to ciliation were seen with a low power. C was seen to pulsate spontaneously. Possibly it did yesterday but it was not watched closely. A piece of the subumbral surface of C broken off (not from the margin) was found to contract spontaneously.