Professor Jacob, late Professor of Pathology, Yorkshire College, Leeds, gives the following example on a large scale, to show the results of insufficient ventilation: "A great politician was expected to make an important speech. As there was no room of sufficient dimensions available in the town, a large courtyard, surrounded with buildings, was temporarily roofed over, some space being left under the eaves for ventilation. Long before the appointed time several thousand people assembled, and in due course the meeting began; but before the speaker got well into his subject, there arose from the vast multitude a cry for air, numbers of people were fainting, and every one felt oppressed and well-nigh stifled. It was only after some active persons had climbed on the roof and forcibly torn off the boards for a space about twenty feet square that the business of the meeting could be resumed." Remembering that the process of breathing is for the purpose of supplying oxygen to the blood and that the absorption of oxygen in the lungs is the same process which goes on when a candle burns, the following experiments were made by Professor King of the University of Wisconsin, to show the effect of expired air on a candle flame. He took a two-quart mason jar and lowered a lighted candle to the bottom, noting that the candle burned with scarcely diminished intensity. Through a rubber tube, he breathed gently into the bottom of the jar, with the result that the candle gradually had a reduced flame and was finally extinguished. He observed also that if the candle were raised as the flame showed signs of going out, the brilliancy of the flame was restored, while lowering the candle tended to extinguish the flame. Even when the candle was raised to the top of the jar, the flame was extinguished after sufficient air had been breathed into the jar. Clearly, then, he argued, air once breathed is not suitable for respiration, unless much diluted with pure air. He argued from this that if a candle using oxygen for combustion could not burn in expired air, therefore an individual using oxygen for the renewal of the blood could not be properly supplied in a room partially saturated with the expired products of the lungs. Professor King also experimented with a candle burning in a jar on which the cover had been placed, and found that the candle was extinguished in thirty seconds, and he argued that if a candle was thus extinguished on account of the carbonic acid given off, so a person shut up in an air-tight chamber would similarly be extinguished in the course of time. To prove that expired air is poisonous to animal life, Professor King experimented on a hen, placing the same in a cylindrical metal air-tight chamber eighteen inches in diameter and twenty inches deep. The hen became severely distressed for want of ventilation and died at the end of four hours and seventeen minutes.