Dr. Marsh’s book cannot fail to promote the practical study of histology wherever the English language is spoken, and under ordinary circumstances the students of this country might have been left to the use of the London edition. But a careful reading showed that one or two points which are perfectly clear to British readers might be a source of doubt and difficulty to students on this side. At the present day in this country histology is a favorite study with very many private students who have no access to teachers or large libraries, and for the purpose of smoothing their path we have added such information as was obviously desirable. In the hope that these additions will prove of value, we submit the book to American readers. NEW YORK, _January, 1879_. ------------------------------------------------------------------------ CONTENTS. ------- PART I. Introduction 11 On Cutting Unprepared Vegetable Tissues 12 On Cutting Unprepared Animal Tissues 13 Preparation of Vegetable Tissues 15 Preparation of Animal Tissues 16 Special Methods of Hardening 19 Cutting Hardened Tissues by Hand 19 Microtome 20 Æther Microtome 24 Section-Knife 24 Imbedding in Paraffine for Microtome 26 Employment of Microtome 29 Staining Agents 31 Carmine Staining 32 Mounting Media 36 Mounting in Glycerine 38 Uses of Freezing Microtome 42 Employment of Freezing Microtome 43 Logwood Staining 47 Absolute Alcohol 50 Clove Oil 51 Canada Balsam 51 Mounting in Balsam 53 Finishing the Slide 55 PART II.