Section-Cutting: A Practical Guide to the Preparation and Mounting of Sections for the Microscope, Special Prominence Being given to the Subject of Animal SectionsMarsh, Sylvester
Science
Section-Cutting: A Practical Guide to the Preparation and Mounting of Sections for the Microscope, Special Prominence Being given to the Subject of Animal Sections
Marsh, Sylvester
Microscopy; Microscopy -- Technique
It will now be necessary to remove any superfluous
glycerine which may have collected around and near the cover. The great
bulk must be wiped away by means of a camel’s-hair pencil, slightly
wetted between the lips, any remaining stickiness being removed with a
bit of blotting paper which has been slightly damped. With a very small
camel’s-hair pencil, charged with solution of gelatine, a ring must be
made round the margin of the cover, of sufficient breadth to take in a
small tract of both cover and slide. As this cement is perfectly
miscible with glycerine, it readily unites with any of that fluid which
may ooze from beneath the cover, and which, in the case of any of the
ordinary varnishes, would act as a fatal obstacle to perfect adhesion.
To make the cement, take half an ounce of Nelson’s opaque gelatine, put
in a small beaker, add sufficient cold water to cover it, and allow the
mixture to remain until the gelatine has become thoroughly soaked. The
water is now poured off, and heat applied until the gelatine becomes
fluid, when three drops of creosote should be well stirred in, and the
fluid mixture transferred to a small bottle to solidify. Before use,
this compound must be rendered liquid by immersing the bottle containing
it in a cup of warm water. When the ring of gelatine has become quite
set and dry (which will not take long), every trace of glycerine must be
carefully removed from the cover and its neighborhood, by gently
swabbing these parts with a large camel’s-hair pencil dipped in
methylated spirit. After drying the slide a ring of Bell’s microscopical
cement may be applied over the gelatine, and, when this is dry, another
coat is to be laid on. If it be desired to give the slide a neat and
tasteful appearance, it is a very easy matter, by means of the
turn-table, to lay on a final ring of Brunswick black or white zinc
cement (§ 24). Every care has now been taken to render our preparation
permanent; but, to make assurance doubly sure, it will be well to follow
Dr. Carpenter’s advice, and, every year or so, to lay on a thin coating
of good gold-size.[12]
Footnote 10:
The appearance of a slide is vastly improved if the preparation be
placed _exactly_ in its centre. This may readily be done in the
following manner:—Take some very finely-powdered Prussian blue, and
rub it up in a mortar with a little of the weak gum solution (§ 11),
so as to form a thin blue pigment. A quantity of this should be made,
so as always to be at hand. A slide having been cleaned, the _best
surface_ is to be selected, and on the _reverse_ side, by means of the
self-centring turn-table, a small circle is to be drawn with a
camel’s-hair pencil, charged with the pigment. In the centre of this
ring, but on the opposite side of the slide, the section is to be
placed, when it of course will occupy a position exactly central. When
the slide comes to be finished, the blue ring may easily be removed
with a wet rag.
Footnote 11:
Public-domain text, read in full here on John Shaqi.
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