Section-Cutting: A Practical Guide to the Preparation and Mounting of Sections for the Microscope, Special Prominence Being given to the Subject of Animal SectionsMarsh, Sylvester
Science
Section-Cutting: A Practical Guide to the Preparation and Mounting of Sections for the Microscope, Special Prominence Being given to the Subject of Animal Sections
Marsh, Sylvester
Microscopy; Microscopy -- Technique
24. _Finishing the Slide._—In the course of two or three days it will be
advisable to take an old penknife, and after heating the blade in the
flame of a spirit lamp, gently to run the point of it round the margin
of the cover, so as to remove any excess of balsam which may have oozed
from beneath it. In a few days more, any remaining balsam may be
carefully scraped away with a cold knife. All remaining traces of balsam
are then to be removed from around the cover by means of a rag _just
moistened_ with methylated spirit, or, what is better, with a mixture of
equal parts of spirit and æther, after which the slide is to be
thoroughly washed in cold water. The slide is now in reality finished,
but, in order to give it a smart appearance, it is usual, with the
assistance of the turn-table, to run a ring of colored varnish round the
covering glass. A very useful varnish for the purpose is the _white zinc
cement_. To prepare this, dissolve an ounce of gum dammar in an ounce of
turpentine by the aid of heat. Take one dram of oxide of zinc and an
equal quantity of turpentine; rub them up together in a mortar, adding
the turpentine drop by drop, so as to form a creamy mixture perfectly
free from lumps or grit. One fluid ounce of the dammar solution
previously made must now gradually be added, the mixture being kept
constantly stirred (_Frey_). The cement, when made, should be strained
through a piece of fine muslin, previously wetted with turpentine, into
a small wide-necked bottle, which, instead of having a cork or stopper,
should be covered with a loose metal cap. Instead of a bottle, the
varnish may be kept in one of the collapsible tubes used by artists; but
though this plan is highly recommended by many, it is not without its
disadvantages. If the varnish becomes thick by keeping, a few drops of
turpentine or benzole well stirred in will soon reduce it to a suitable
consistence for use.
------------------------------------------------------------------------
PART II.
25. _Special Methods._—Having in the preceding pages entered at some
length into the general subject of section-cutting, it remains for us
now to consider those special methods of preparation which the
peculiarities of certain objects demand. In order to keep the bulk (and
consequent price) of this manualette within due bounds, we shall,
without further preface, proceed to the description of these methods, in
doing which every endeavour will be made to employ such brevity of
expression as may be consistent with perfect clearness of meaning. As
the most convenient plan, the objects here treated of will be arranged
in alphabetical succession.
Public-domain text, read in full here on John Shaqi.
Reviews
Reviews
No reviews yet
Be the first to share your thoughts on this work.
Join the Discussion
Join the discussion
Sign in to leave a comment or review.
Sign InorCreate an account