Section-Cutting: A Practical Guide to the Preparation and Mounting of Sections for the Microscope, Special Prominence Being given to the Subject of Animal SectionsMarsh, Sylvester
Science
Section-Cutting: A Practical Guide to the Preparation and Mounting of Sections for the Microscope, Special Prominence Being given to the Subject of Animal Sections
Marsh, Sylvester
Microscopy; Microscopy -- Technique
26. _Bone._—Both transverse and longitudinal sections should be
prepared, the former being the prettier and most interesting. After
prolonged maceration in water, all fat, etc., must be removed and the
bone dried, when as thin a slice as possible is to be cut off in the
desired direction, by means of a very fine saw. If the section so
obtained be placed upon a piece of smooth cork it may, with the aid of a
fine file and the exercise of care, be further reduced in thickness. It
is then to be laid upon a hone moistened with water, and being pressed
gently and _evenly_ down upon it with the tip of the finger (protected,
if necessary, by a bit of cork or gutta-percha), it must be rubbed upon
the stone until the desired degree of thinness has been attained.
Finally, in order to remove scratches and to polish the section, it
should be rubbed upon a dry hone of very fine texture, or upon a strop
charged with putty-powder. After careful washing in several waters the
section must be allowed thoroughly to dry, when it may be mounted by the
_dry method_ in the following manner:—A ring of gold-size must, by means
of the turn-table, be drawn in the centre of a slide, and the slide put
away in a warm place for several days (the longer the better), in order
that the ring may become perfectly dry and hard. When this has been
accomplished the section is to be placed in the centre of the ring, and
a covering circle of the requisite size having been cleaned, this must
have a _thin_ ring of gold-size applied round its margin. The cover is
now to be placed in position and gently pressed down, a spring clip
being employed, if necessary, to prevent it from moving. In about
twenty-four hours another layer of the varnish should be applied, and
the slide afterwards finished in the manner already described (§ 24).
The above method is also applicable to the preparation of sections of
_teeth_ and also of _fruit-stones_ and other hard bodies, which are
incapable of being rendered soft enough for cutting.
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