Section-Cutting: A Practical Guide to the Preparation and Mounting of Sections for the Microscope, Special Prominence Being given to the Subject of Animal Sections — John Shaqi
Section-Cutting: A Practical Guide to the Preparation and Mounting of Sections for the Microscope, Special Prominence Being given to the Subject of Animal SectionsMarsh, Sylvester
Science
Section-Cutting: A Practical Guide to the Preparation and Mounting of Sections for the Microscope, Special Prominence Being given to the Subject of Animal Sections
Marsh, Sylvester
Microscopy; Microscopy -- Technique
It is well, in connection with these papillæ, to bear in mind a fact
pointed out by _Frey_, namely, that the tips of the fingers frequently
become, _post-mortem_, the seat of extensive natural injections;
hence, in sections from this region, we frequently obtain good views
of distended capillaries without having been at the trouble of
previously injecting them.—_Frey_, “Microscopical Technology.”
43. _Spinal Cord._—The spinal cord, say of a cat or a dog (or if
procurable, of man), after being cut into pieces about half an inch in
length, may be hardened in the usual chromic acid fluid (§ 5). As it is
peculiarly liable to overharden and become uselessly brittle, the
process must be carefully watched. Its further treatment is the same as
that of brain. These sections may be stained very satisfactorily by the
_ink process_, for communicating details of which we are indebted to the
kindness of Dr. Paul, of Liverpool. The agent usually employed is
Stephenson’s blue-black ink, which, for this purpose, must be quite
fresh. As in the case of carmine, two methods of staining may be
adopted—either rapid, by using concentrated solutions, or more
prolonged, according to degree of dilution. For the reasons previously
given (§ 14), slow methods of staining are always to be preferred, as
yielding the most beautiful results, yet, for the purposes of
preliminary investigation, it is often convenient to have recourse to
the quick process. To carry out the latter plan, an ink solution of the
strength 1 in 5—10 parts of water is to be freshly prepared, and the
sections exposed to its action for a few minutes. For gradual staining
the dilution must be carried to 1 in 30—50, and the time of immersion
prolonged to several hours, the sections being occasionally examined
during the staining, so that they may be removed just as they have
acquired the desired tint. When a satisfactory coloration has been
obtained, the preparations should be mounted in dammar or balsam (§ 23).
One advantage of this method of staining is, that definition is almost
as good by artificial light as by day.
44. _Sponge_ may readily be cut after being tightly compressed between
two bits of cork; or its interstices may be filled up by immersion
either in melted paraffine (§ 11) or in strong gum (§ 18), and then cut
as usual.
45. _Stomach_ requires no special method of hardening (chromic acid).
Sections should always, when practicable, be cut in the freezing
microtome. In default of this, proceed in the manner as directed for
lung (§ 35). Both vertical and horizontal sections will, of course, be
required. If the preparation has been injected, the latter are
particularly beautiful. Stain with carmine or aniline blue (§ 27), and
mount—if for very close study, in glycerine—if injected and for a “show”
slide, use balsam.
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