Section-Cutting: A Practical Guide to the Preparation and Mounting of Sections for the Microscope, Special Prominence Being given to the Subject of Animal SectionsMarsh, Sylvester
Science
Section-Cutting: A Practical Guide to the Preparation and Mounting of Sections for the Microscope, Special Prominence Being given to the Subject of Animal Sections
Marsh, Sylvester
Microscopy; Microscopy -- Technique
46. _Tongue._—Harden in chromic acid, imbed and cut transverse sections
in paraffine. As, however, the paraffine is apt to get entangled amongst
the _papillæ_, whence it is afterwards with difficulty dislodged, it
will be as well before imbedding to soak the tongue in strong gum for a
_few minutes_, and afterwards immerse in alcohol till the gum becomes
hardened, so that the delicate papillæ may thus be protected from the
paraffine by a surface-coating of gum. The best staining agent is
picro-carmine (§ 42). Sections of _cat’s_ tongue near the root, when
thus stained, furnish splendid objects. Sections should also be made of
the _taste-bulbs_, found on the tongues of rabbits. These are small oval
prominences, situated one on each side of the upper surface of the
tongue near its root. They should be snipped off with scissors, and
vertical sections made in the direction of their long axis. Stain with
carmine or picro-carmine, and mount in glycerine or balsam.
47. _Vegetable Ivory_.—After prolonged soaking in cold water may readily
be cut in the microtome. The sections should be mounted in balsam, and
though not usually regarded as polariscopic objects, nevertheless, when
examined with the _selenite_, yield very good colours.
48. _Wood._—Shavings of extreme thinness may be cut from large pieces or
blocks of timber, by means of a very sharp plane. In this way very good
sections may be procured of most of the common woods, as oak, mahogany,
“glandular wood” of pine, etc. Where however, the material to be
operated upon takes the form of stems, roots, etc., of no great
thickness, they should, after having been reduced to a suitable
consistence (§ 4), be imbedded in paraffine, and cut in the microtome.
Before imbedding it must not be forgotten to immerse the wood to be cut
in weak gum-water (§ 11), this precaution being of great importance,
especially in the case of stems, etc., the bark of which is at all rough
and sinuous. If the sections are to be mounted _unstained_, they are
usually put up in weak spirit (§ 26). A very general method also of
dealing with this class of objects is to mount them _dry_ (§ 26). This
plan, however, cannot be recommended, for however thin the sections may
be, the outlines, when this process is adopted, always present a
disagreeable black or blurred appearance. To avoid this we may have
recourse to Canada balsam, but the ordinary method of employing it must
be slightly modified, a drop of chloroform being substituted for the
clove oil (§ 23), otherwise this latter agent will cause the section to
become so transparent as to render minute details of structure difficult
to recognize. A better plan, perhaps, is to stain the section with
carmine or logwood, and mount in balsam by the ordinary process. The
best course to follow, however, especially in the case of transverse
sections, is the _double staining_ method.[18] For this purpose the
sections in the first place must be subjected to the action of a
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