Section-Cutting: A Practical Guide to the Preparation and Mounting of Sections for the Microscope, Special Prominence Being given to the Subject of Animal Sections — John Shaqi
Section-Cutting: A Practical Guide to the Preparation and Mounting of Sections for the Microscope, Special Prominence Being given to the Subject of Animal SectionsMarsh, Sylvester
Science
Section-Cutting: A Practical Guide to the Preparation and Mounting of Sections for the Microscope, Special Prominence Being given to the Subject of Animal Sections
Marsh, Sylvester
Microscopy; Microscopy -- Technique
RANGOON oil is not found in this market. It is a variety of naphtha or
petroleum found at Rangoon, in Asia, and contains a variable percentage
of solid paraffin. Kerosene with a very little paraffin dissolved in it,
would undoubtedly answer the same purpose.
Good sperm oil or tallow (free from salt) will serve quite as well. It
is scarcely necessary to caution the reader that all oily matter should
be removed from the knife before it is used for cutting sections. This
is best effected by dipping it in hot water and carefully wiping it.
NOTE B., Page 15.
METHYLATED spirit is alcohol which contains a small percentage of
methyl. This renders it unfit for use in the manufacture of liquors or
even chloroform, but does not injure it as a preservative fluid. The
scientific men of Great Britain have the privilege of obtaining this
fluid free from duty, and consequently they make very general use of it.
In this country the student of science has no such privilege, and must
use the more expensive alcohol. This however is but of a piece with
other restrictions placed upon scientific pursuits in this country,
where men like Marshall Jewell and Hannibal Hamlin have influence enough
to pass laws which prohibit the exchange by mail of microscopic
specimens, which pass freely in every other civilized country.
True methyl alcohol, or methylated spirits, is not to be had in this
country, and therefore whenever this liquid is directed to be used in
English books the American microscopist must substitute good alcohol.
What is sold for “methyl alcohol” is wood spirit or wood naphtha, and
will not serve the purposes of the microscopist, though it answers well
enough for spirit lamps, making varnishes, etc.
NOTE C., Page 20.
THE method described in the text is generally known as the “free-hand”
method, and although it is not generally employed for the production of
cabinet specimens or slides that are intended for sale, yet it possesses
so many advantages that every student should endeavor to acquire the art
of cutting sections without the use of the microtome. For purposes of
study such sections are quite equal to the best of those usually found
on sale, and for the encouragement of beginners we may add that we have
seen sections cut by the free-hand method which for size, thinness and
accuracy, excelled any that we have ever seen produced with the
microtome. Indeed Schäffer distinctly affirms that no microtome can
equal the skilled hand in the production of thin sections.
NOTE D., Page 22.
THE mere existence of a binding screw should be no objection, and where
bushings are employed it is sometimes necessary. But as the author well
observes, a binding screw cannot be used to secure histological
preparations.
NOTE E., Page 22.
Public-domain text, read in full here on John Shaqi.
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