Transcriber’s notes: Apart from the following corrected misspellings the text of this book has been preserved as in the original: xolol —> xylol side —> slide overstraining —> overstaining Where appropriate, illustrations and footnotes have been positioned adjacent to the relevant text. SECTION CUTTING AND STAINING A PRACTICAL INTRODUCTION TO HISTOLOGICAL METHODS FOR STUDENTS AND PRACTITIONERS BY W. S. COLMAN, M.D., M.R.C.P. ASSISTANT PHYSICIAN (FORMERLY PATHOLOGIST) TO THE NATIONAL HOSPITAL FOR THE PARALYSED AND EPILEPTIC; AND TO THE HOSPITAL FOR SICK CHILDREN, GREAT ORMOND STREET, ETC. SECOND EDITION _ENLARGED AND IN MOST PART RE-WRITTEN_ LONDON H. K. LEWIS, 136 GOWER STREET, W.C. 1896 PRINTED BY H. K. LEWIS, 136 GOWER STREET, LONDON, W.C. PREFACE TO THE SECOND EDITION. In preparing this edition I have endeavoured to meet the requirements of students, and of practitioners who desire to keep up their histological work. Those methods are selected which have been found to work well in practice, and it has been thought better to describe a few in detail rather than give a short account of many similar methods. I have again to express my obligation to the various instrument makers for the illustrations of microtomes, &c.; to Dr. Fearnley, of Bradford, for the description of his method for injecting blood vessels, and to Messrs. Macmillan and Co. for permission to copy figures 10 and 11. W. S. COLMAN. Wimpole Street, W. _Sept., 1896._ CONTENTS. PAGE CHAPTER I. Apparatus Required 1 CHAPTER II. Hardening Processes 15 CHAPTER III. Section Cutting 29 CHAPTER IV. Section Mounting 55 CHAPTER V. General Staining Methods 67 CHAPTER VI. Special Methods for Staining the Nerve Centres 87 CHAPTER VII. Special Methods for Staining Micro-Organisms and Blood 103 CHAPTER VIII. Injection of Blood Vessels 120 CHAPTER IX. Directions for Preparing Individual Tissues 129 Index 153 SECTION CUTTING AND STAINING. CHAPTER I. APPARATUS REQUIRED. Probably there is nothing more perplexing to a beginner than to decide what apparatus is required. If he consult a price list, it is difficult for him to tell which articles will be necessary, and which will be either luxuries, or required only for special investigation.