Section Cutting and Staining: A practical introduction to histological methods for students and practitioners — John Shaqi
Section Cutting and Staining: A practical introduction to histological methods for students and practitionersColman, Walter S.
Science
Section Cutting and Staining: A practical introduction to histological methods for students and practitioners
Colman, Walter S.
Microscopy
In the following account of requisites, those only will be described
which it is useful to have always at hand. They will be found
sufficient for ordinary work, but for special investigations a more
elaborate equipment will be required.
All staining and other reagents should be made as far as possible
by the worker himself, according to the directions given in later
chapters. This should at any rate be done at first, as the knowledge
thus gained will prove invaluable. It will also effect a great saving
if articles that are used in any quantity, such as methylated spirit,
distilled water, &c., are bought by the gallon, and not in small
quantities.
Almost all the processes described here can be carried out without the
use of a fully equipped laboratory, in fact, in an ordinary room. The
only furniture required is a firm table, and a cupboard and shelves for
storing reagents.
The following should also be procured:--
*Jars* or *bottles*, with well fitting stoppers or corks, to contain
the tissues while being hardened. They should not hold less than two
ounces. Empty drug bottles which can usually be obtained from druggists
for a few pence, serve very well.
Smaller bottles should also be procured for keeping specimens in spirit
after they have been hardened until one is ready to cut sections. After
sections have been cut from a portion of the specimen, the rest should
be preserved, in case it is wanted for further investigation. Each
specimen must be labelled, with a name or a number corresponding to a
reference in the note-book, and a large number of specimens may then
be kept in the same jar. The best way to label them is to write the
name or number on a piece of vegetable parchment in ordinary “marking
ink,” and warm it until the writing is black. The little label should
then be fixed to a corner of the piece of tissue with a stitch or by a
fine pin, and it may be identified years afterwards. The importance of
keeping tissues, sections, slides, &c., *distinctly labelled* cannot
be too strongly impressed on the beginner. The name, date, and other
particulars should be invariably written on the label at the time.
At first the student will be inclined to neglect this, as he will
recognize his pieces of tissue and sections so readily merely by their
shape and general appearance. But as time elapses and similar specimens
accumulate, he will find it most difficult or even impossible to
identify one from the other.
A number of 1 oz. and 2 oz. *stoppered bottles* for staining reagents.
The stopper of these should be fitted with a rod. This is done by
simply heating the lower end of the stopper and the upper end of a
piece of glass rod of suitable length in a blow-pipe, until they are
plastic, and then pressing them together.
*Watch glasses.*--At least a dozen watch-glasses, in which to perform
the operations of staining, clarifying, &c. Those with a flat bottom
should be employed as they are less easily upset than the others.
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