Section Cutting and Staining: A practical introduction to histological methods for students and practitioners — John Shaqi
Section Cutting and Staining: A practical introduction to histological methods for students and practitionersColman, Walter S.
Science
Section Cutting and Staining: A practical introduction to histological methods for students and practitioners
Colman, Walter S.
Microscopy
Plenty of *filter papers*.
Both coarse ones, for use in the manufacture of reagents, and small
fine white ones (2-1/2 inch) for filtering the staining fluids
immediately before using them, should be procured. Before using them
a few drops of alcohol or distilled water should be placed in them to
saturate the paper. This not only allows the fluid to pass through
more rapidly, but prevents a portion of it being wasted through being
absorbed by the pores of the paper.
Several *needles* mounted in handles.
They must be kept very bright and smooth, and care must be taken that
the point does not get turned up.
A large and small *funnel*.
Several *pipettes* consisting of pieces of glass tube with an internal
diameter of 1/8″ and about ten inches long, drawn out almost to a point
at one end.
*Section lifter.*--This instrument is required for transferring
sections from one reagent to another, or from oil of cloves, &c., to
the slide. The most convenient form is Woodhead’s, made of thin sheet
copper, which allows the blade to be bent at any angle to the stem. The
stem or handle is about six inches long, and continuous with, and at an
angle to it, a flat blade about 3/4 in. square with the corners rounded
off. Larger ones can be obtained for mounting sections of large size,
_e.g._, kidney, medulla oblongata, &c. The surface of the blade should
be brightly polished, and kept scrupulously clean.
Ordinary dissecting *forceps*.
One or two *scalpels*.
A pair of fine *scissors*.
A *razor* or other instrument for cutting sections.
A smooth *oil stone* for keeping the razors and knives properly
sharpened.
A *spirit lamp* for warming the staining fluids.
A few *test tubes*.
A *minim measure*.
*Scales* and small weights.
A gross of ground glass slides 3 x 1 in.
Half a gross of ground glass slides 3 x 1-1/2 in.
Half an ounce of thinnest coverslips, 7/8 in. diameter.
Quarter of an ounce of thinnest coverslips, 1-1/4 in. diameter.
*Microscope.*--This is not the place for a description of the
microscope as an optical instrument, but some hints as to the selection
of one may be found useful.
Showy microscopes with much brass work should be avoided, simplicity of
construction being a great recommendation. The microscope should have
a large heavy base, either of the horse-shoe or tripod pattern, large
enough to afford a firm base when the microscope is tilted.
Mechanical stages are unnecessary and they add greatly to the expense,
and very little to the utility of the instrument for ordinary
histological work. Binocular arrangements also are of little use for
this purpose.
The microscope should be provided with a *coarse and fine adjustment*,
which should be most carefully tested before purchasing the instrument.
They should work freely and smoothly, and the slightest turn in either
direction should at once alter the focus.
Public-domain text, read in full here on John Shaqi.
Reviews
Reviews
No reviews yet
Be the first to share your thoughts on this work.
Join the Discussion
Join the discussion
Sign in to leave a comment or review.
Sign InorCreate an account