Balsam in benzol and glycerine jelly are desirable for mounting media,
and some wax sheets will be needed for making cells. In addition, the
analyst should supply himself with specimens of whole spices, starches,
and known adulterants, which may be used to become acquainted with the
forms and appearances to be expected; it is easier to begin one’s study
in this way on sections prepared with the knife, and afterwards the
powdered substance may be taken up.
To study the physiological structure in the spices and their adulterants
is quite difficult, as the vegetable tissues which make up the structure
of the spices and the materials of a vegetable origin which are added as
adulterations consist of cells of different forms and thickness; those
which are most prominent and common are the parenchyma, the sclerenchyma
fibrous tissue, and the fibro-vascular bundles. Spiral and dotted
vessels are also common in several of the adulterants, and in the
epidermis are other forms of tissue which it is necessary to be well
acquainted with, though not physiologically. The parenchyma is the most
abundant tissue in all material of vegetable origin, making up the
largest proportion of the main part of the plant. It is composed of thin
wall cells which may be recognized in the potato and in the interior of
the stems of maize. In the latter plant, also, the fibro-vascular system
is well exemplified, running as scattered bundles between the nodes or
joints. Fibrous tissue consists of elongated thick-walled cells of
fibers which are very common in the vegetable kingdom and are well
illustrated in flax, but they are not so commonly used for adulterating
purposes. They are optically active, and in the shorter forms they
somewhat resemble the cells next described. They are seen in one of the
coats of buckwheat hulls and in the outer husks of the cocoanut.
The sclerenchyma is found in the shells of many nuts and in one or two
of the spices, the cells being known as stone cells, from the great
thickening of their walls. To them is due the hardness of the shell of
the cocoanut, the pits of the olive, etc. (See Fig. 1.) Spiral and
dotted vessels are common in woody tissue and are readily recognized.
All these forms an analyst should make himself familiar with.
In pepper and mustard the parenchyma cells are prominent in the interior
of the berry, while those constituting the outer coats are indistinct in
the pepper, because of their deep color; but in the mustard are
characteristics of this particular species. In fact, in many of the
spices, and especially those which are seeds, the forms of the epidermal
cells are very striking, and, if no attempt is made to classify them
their peculiarities must be carefully noted, as the recognition of the
presence of foreign husky matter depends upon a knowledge of the normal
appearance in any spice.
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