After having completed the first dissection (the hyaloid, contents and attachments), and the specimen has been in the formaldehyde solution for ten days or two weeks, it will have become hard and tough enough to stand a considerable amount of rough handling. If the specimen has been kept in a large receptacle, such, for instance, as a jar, remove it with a spoon; if in a small jar or vial, empty out the fluid, then slide the specimen out on whatever has been prepared to receive it. Turn it so that the lens will be uppermost. Find the suspensory ligament in the Zone of Zinn. Insert the pointed end of the glass tube, close to the lens, and blow gently until the canal shows its sacculated construction by filling with air, giving the appearance of a lot of little bubbles surrounding the periphery of the lens. (Fig. 8.) It may be necessary to move the blowpipe in and out in order to find the canal, all the while blowing steadily through the tube. THE INTERIOR OF THE EYE For the study of the interior of the eye and its contents _in situ_ either a fresh or a hardened eye will do; a hardened eye is preferable. In the dissection for isolating the hyaloid membrane, vitreous, lens, and other parts, the anterior and posterior halves of the evacuated eye may be separated entirely, and each half studied. However, the choroid and the retina will be more or less mutilated, and the vitreous and other parts will be removed. The absence of these parts will prevent one from receiving a definite idea of their anatomical relationships. Therefore, it is better to work with an entire and complete eye. [Illustration: Fig. 9--Showing method of cutting eye into anterior and posterior sections with safety-razor blade.] Remove all the muscles and fatty tissues from the outside of the globe; then cut it in half through the equator, thus dividing it into an anterior and a posterior half. The cutting of the sclerotic, as well as the underlying tissues and the vitreous, should be done with the large scissors; using a knife or scalpel will tend to disturb the positions of those tissues or so tear them that they will not be of much use for purposes of study. An ordinary safety-razor blade makes an excellent instrument for separating the eye into two halves, because it cuts through the tissues without tearing them in any way. (Fig. 9.) The rather dark colored, viscid fluid that escapes when the eye is halved is the perichoroidal lymph, not the aqueous, as is sometimes stated. THE POSTERIOR HALF The posterior half is taken first because it is the simplest and easiest of the two halves to dissect. In this half of the eye the retina may be readily seen through the vitreous; the choroid and its apparent iridescent colors through both vitreous and retina. (Fig. 10.) Remove the vitreous by simply tilting this half of the eye, and with the finger push out the vitreous.