Technique of Eye DissectionsWoll, Frederic A. (Frederic Albert)
Science
Technique of Eye Dissections
Woll, Frederic A. (Frederic Albert)
Dissection; Eye -- Surgery
[Illustration: Fig. 10--The retina, retinal vessels, and iridescent
choroid showing through the vitreous.]
Sometimes the vitreous will adhere very closely to the retina. This
occurs especially when the eye has been in formaldehyde for a long time.
In such a case the removal of the vitreous without injuring the retina
requires patience and care. The use of the scalpel and the scissors may
become necessary. Another very good way to remove the vitreous is to
take hold of the sclerotic, turn it so that the vitreous is downward,
and then shake gently until the vitreous separates itself from the
retina and, drops out. After the vitreous has been removed, notice its
glassy appearance; hence its name--hyaloid body. Try to pull it apart
with the fingers, and it will be noticed that it seems to be held
together by more or less of a network of fibres. (Fig. 11.)
[Illustration: Fig. 11--Showing how vitreous seems to be held together
by a network of fibres. (Page 41.)]
Whichever method for removing the vitreous is followed, the retina will
be left rather badly wrinkled and out of place. If the last-mentioned
method, which is really the best of the three described, is the one
adopted, the retina will be left in an entirely collapsed and folded
form. In any case, to straighten out the retina against the choroid,
immerse the whole posterior half in water, inside uppermost. The retina
will then slowly unfold itself and lie flat against the choroid. With
the tweezers remove the whole half from the water; tilting it slowly to
empty it of all the water, and, having done so, turn it down upon the
table rather forcibly in order to help it drain itself of all the water.
Notice the thinness of the retina, and, also, that the seeming
iridescence of the choroid shows through. The optic disc, which is the
point of entrance of the optic nerve, and the optic cup are easily
recognized, though neither will be seen as large as when viewed in the
living eye with an ophthalmoscope. The blood vessels of the retina, as
they ramify outward or forward, after their entrance through the optic
nerve through which they pass, are also very plainly seen. A closer
inspection will show, in the very centre of the “entrance” of the optic
nerve, a whitish, pointed vessel, about 1 or 2 mm. long. That is the
sloughed-off and atrophied end of the hyaloid artery, which, when the
eye was in an embryonic state, ran forward from the central artery of
the retina through the hyaloid canal to the posterior surface of the
lens. With the forceps pick up the peripheral edge of the retina, and,
by pulling gently upward, tear it away from its apparent place of
attachment to the “entrance” of the optic nerve. (Fig. 12.) When this
has been done, there will be seen some threads protruding from the
optic nerve. Filling the half with water will tend to separate these
strands, which are optic-nerve elements.
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