Technique of Eye DissectionsWoll, Frederic A. (Frederic Albert)
Science
Technique of Eye Dissections
Woll, Frederic A. (Frederic Albert)
Dissection; Eye -- Surgery
After the choroid and the sclerotic have been cut away, drop the retina
into some water, and it will slowly unfold itself by “ballooning” out
into a perfect and beautiful specimen. (Fig. 40.) But, if it is desired
to study the specimen closely, it is better to suspend it in a jar or
bottle made of thin glass, and containing a 5 per cent. solution of
formaldehyde. Remember that the retina is a delicate membrane in any
state; the slightest rough handling may cause it to be torn, or
otherwise damaged. If the vessel, in which the specimen has been placed
and suspended, has enough preserving fluid to completely fill it, and it
is firmly stoppered, the whole thing may be inverted, and turned in any
direction, even abruptly, without fear of damaging the retina. This way
of keeping the retina will give opportunity to inspect and study the
inside as well as the outside of the membrane; the blood-vessels, and
other important parts easily recognized.
THE SAGITTAL OR VERTICAL SECTION OF THE EYE
Place an eye in a 5 per cent. solution of formaldehyde for about two
weeks. If the eye is kept in that solution longer than that time, the
lens is apt to become so hard that in cutting it the capsule and
suspensory ligament will be torn, and the lens will then become
detached; if for a shorter space of time, the lens and other tissues
will be so soft that all may be so badly torn or lacerated, that a
perfect specimen will not be possible.
It sometimes happens that in keeping a number of eyes together in a
vessel for the purpose of hardening them in the formaldehyde solution,
the corneas of some will be crushed in. For this dissection, select an
eye that has the cornea in perfect condition.
Remove all the outside tissues with the scissors, being particular to
have the region immediately surrounding the optic nerve perfectly clear
and clean. If the optic nerve is longer than 5 mm., cut it off to that
length.
[Illustration: Fig. 41--Showing the beginning of the cutting of the eye
for sagittal sections.]
[Illustration: Fig. 42--Showing method of cutting through the
crystalline lens.]
To cut the eye in two, use a safety-razor blade; never a scalpel. The
latter is too thick, too dull, and too clumsy a tool. Begin by cutting
through the optic nerve; dividing it as nearly as possible into halves.
(Fig. 41.) Continue cutting through the sclerotic and all underlying
tissues, stopping at the corneo-scleral junction, but do not, during
this procedure, even touch the lens. After the eye has been thus partly
separated into, as nearly as possible, two equal parts, lay it down upon
the cornea, and, holding the razor blade in the forefingers and thumbs
of both hands, cut the lens in two by forcing the blade down through it.
(Fig. 42.) Partly open the cut eye to allow one jaw of the large
scissors to enter, turn the eye over so the cornea will rest on that
jaw, and then cut through the cornea. (Fig. 43.)
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