Technique of Eye DissectionsWoll, Frederic A. (Frederic Albert)
Science
Technique of Eye Dissections
Woll, Frederic A. (Frederic Albert)
Dissection; Eye -- Surgery
Procure the eye of either a sheep or a bullock. Instead of following the
usual procedure of hardening in any one of the several solutions used
for the purpose of toughening the ocular tissues, place the eye in a
cool place and permit it to collapse a trifle. Usually two or three days
is a sufficient length of time to accomplish the result.
Experiments have shown that if an eye is too fresh the ciliary
processes will not be easily detached from the hyaloid (zonular
processes), and if the eye has been in a preserving fluid, the same
result will follow. A sheep’s eye will make a better specimen even if it
is small, because the ciliary processes are more easily separated from
the zonular processes. If a bullock’s eye is used, it must be left in a
cool place a day or two longer than in the case of a sheep’s eye, in
order to permit a long enough time to elapse to allow disintegration of
the eye to take place sufficient to have the two processes separate
easily and cleanly.
[Illustration: Fig. 2--Making the first cut. (Page 27.)]
[Illustration: Fig. 3--Showing how the point of the lower jaw of the
scissors is to be kept away from the underlying tissues. (Page 27.)]
[Illustration: Fig. 4--Showing about half of the sclerotic separated.
(Page 29.)]
With a pair of dissecting forceps pinch up the sclerotic about 5 mm.
anterior to the equator. With a pair of small, fine-pointed scissors,
make an incision. (Fig. 2.) Next hold the eye in the left hand without
exerting any pressure. Insert the point of the scissors into the
incision which has been made, and cut. Be careful to keep the point of
the scissors close to the sclerotic or an untimely puncturing of the
choroid will occur. (Fig. 3.) Continue the cutting on a line parallel
to the equatorial meridian and about 5 mm. anterior to it until about
half the sclerotic has been separated. In cutting, always move the point
of the scissors forward with a slight oscillating lateral movement.
(Fig. 4.) While doing this, partly suspend the eyeball from the point of
the scissors. Doing these things will tend to loosen the choroid from
the sclerotic and prevent puncturing too soon the former mentioned
membrane. Now apply pressure in such a manner that the lips of the cut
sclerotic will gap. Into this put the point of the scissors and very
carefully pick up the choroid and the retina with the point of the
scissors and cut them. (Fig. 5.) If the choroid alone has been picked
up and separated, the retina will show milky white or yellowish white
underneath. The retina must then also be separated. Care must be taken
not to go deeper than the retina or the hyaloid may be damaged. Continue
the cutting of the choroid and the retina for a distance of about 20 mm.
Apply enough pressure occasionally so that the vitreous will be forced
upward and above the cut choroid and the retina. This will show whether
any strands of the two membranes have been left uncut. If the separation
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