The ink, too, in which these notes are written illustrates the shifts to which our ancestors were put when writing-materials were not made and bought by the quantity, as they are now,--a fact which bears against a not yet well-established point made by Mr. Maskelyne of the British Museum against Mr. Collier's marginalia. This writing exhibits every possible variety of tint and of shade, and also of consistence and composition, that ink called black could show. As far as the recto of folio 12 it has the look of black ink slightly faded. On the reverse of that folio it suddenly assumes a pale gray tint, which it preserves to the recto of folio 20. There it becomes of a very dark rich brown, so smooth in surface as almost to have a lustre, but in the course of a few folios it changes to a pale tawny tint; again back to black, again to gray, again to a fine clear black that might have been written yesterday, and again to the pale tawny, with which it ends. It is also worthy of notice, that, where this ink has the dark rich brown hue, it also seems, in the words of Professor Maskelyne, in his letter to the London "Times," dated July 13, 1859, to be "on rather than in the paper"; and it also proved in this instance, to use the phraseology of the same letter, to be "removable, with the exception of a slight stain, by mere water." But who will draw hence the conclusion of the Professor with regard to the fluid used on the Collier folio, that it is "a water-color paint rather than ink,"--unless "ink" is used in a mere technical sense, to mean only a compound of nutgalls and sulphate of iron?[aa] [Footnote aa: The effect produced upon the brown ink on the margins of the Guazzo by the mere washing it for a few seconds with lint and warm water may be seen in the word "_apollegy_" on folio 25, reverse, of that volume, which, with the others noticed in this article, will be left for inspection at the Astor Library, in the care of Dr. Cogswell, for a fortnight after the publication of this number of the _Atlantic_. This slight ablution, hardly more effective than the rubbing of a child's wet finger, leaves only a pale yellow stain upon the paper.] Now it should be observed, that, among all the fac-similes published of the marginal readings in Mr. Collier's folio, there are none either so modern or so antique in their character as the five fac-similes respectively given above; nor is there in the former a variation of style approaching that exhibited in the latter, which all surely represent the work of one hand. Neither do the fac-similes of the folio corrections exhibit any chirography more ancient, more "Gothic," than that of the account a specimen of which was published in our previous article upon this subject,[bb] and which could not have been written before 1656, and was quite surely not written until ten years later. [Footnote bb: See the _Atlantic_ for October, 1859, p. 516.] * * * * *