If the pollen which bees transport to their hives has been moistened with some fluid substance which causes the grains to cohere, this addition should be indicated by differences in the results of an analysis of pollen from a plant as compared with that found in the corbiculæ of a bee which has been working on this plant. For the sake of determining this difference and in an endeavor to ascertain, if possible, the approximate nature of the added fluid, analyses were made of three kinds of pollen, as follows: (1) Pollen collected by hand from the corn plant itself; (2) pollen taken from the corbiculæ of bees which had secured their supply from corn; (3) pollen stored in the cells of the hive. In the first two cases pollen from the same species of plant (corn) was used. The material from the cells of the hive was composed largely of corn pollen, but contained an admixture of some other pollens. The writer is indebted to Dr. P. B. Dunbar, of the Bureau of Chemistry, for the following analyses: Pollen Corn pollen Stored direct from pollen from corbicula. from corn. hive. ----------------------------------------------------- Total solids 53.47 66.94 79.66 Moisture 46.53 33.06 20.34 Reducing sugar before inversion 2.87 11.07 17.90 Sucrose 2.77 3.06 2.25 Total reducing sugar after inversion 5.79 14.29 20.27 ===== ===== ===== Dry basis: Reducing sugar 5.37 16.54 22.47 Sucrose 5.18 4.57 2.82 ----- ----- ----- 10.55 21.11 ····· These analyses show conclusively that a very large amount of sugar has been added to the pollen by the time it reaches the corbiculæ. Calculated on a dry basis just about twice as much sugar is present in the basket pollen as in that from the corn plant. Not only is this so, but the additional fact is disclosed that over three times as much reducing sugar is present in the corbicular pollen as sucrose. This latter result indicates that honey (largely a reducing sugar) rather than nectar (containing more sucrose) is the chief sugar ingredient of the corbicular pollen. The additional amount of sugar (here again a reducing sugar) in the stored pollen of the hive is what might be expected, since it is supposed that the workers add honey and possibly other ingredients to the pollen within the storage cells. The total solid percentages, corn 53.47, corbicula 66.94, stored pollen 79.66, also show that the fluid substance which is added is one highly charged with solids, a condition which honey amply fulfills.