Two plants of this variety of Mimulus, belonging to the sixth self-fertilised generation, and growing in separate pots, were intercrossed; and some flowers on the same plants were again self-fertilised. From the seeds thus obtained, plants derived from a cross between the self-fertilised plants, and others of the seventh self-fertilised generation, were raised. But this cross did not do the least good, the intercrossed plants being inferior in height to the self-fertilised, in the ratio of 100 to 110. This case is exactly parallel with that given under Ipomoea, of the grandchildren of Hero, and apparently of its great-grandchildren; for the seedlings raised by intercrossing these plants were not in any way superior to those of the corresponding generation raised from the self-fertilised flowers. Therefore in these several cases the crossing of plants, which had been self-fertilised for several generations and which had been cultivated all the time under as nearly as possible the same conditions, was not in the least beneficial. Another experiment was now tried. Firstly, plants of the eighth self-fertilised generation were again self-fertilised, producing plants of the ninth self-fertilised generation. Secondly, two of the plants of the eighth self-fertilised generation were intercrossed one with another, as in the experiment above referred to; but this was now effected on plants which had been subjected to two additional generations of self-fertilisation. Thirdly, the same plants of the eighth self-fertilised generation were crossed with pollen from plants of a fresh stock brought from a distant garden. Numerous plants were raised from these three sets of seeds, and grown in competition with one another. The plants derived from a cross between the self-fertilised plants exceeded in height by a little the self-fertilised, namely, as 100 to 92; and in fertility in a greater degree, namely, as 100 to 73. I do not know whether this difference in the result, compared with that in the previous case, can be accounted for by the increased deterioration of the self-fertilised plants from two additional generations of self-fertilisation, and the consequent advantage of any cross whatever, along merely between the self-fertilised plants. But however this may be, the effects of crossing the self-fertilised plants of the eighth generation with a fresh stock were extremely striking; for the seedlings thus raised were to the self-fertilised of the ninth generation as 100 to 52 in height, and as 100 to 3 in fertility! They were also to the intercrossed plants (derived from crossing two of the self-fertilised plants of the eighth generation) in height as 100 to 56, and in fertility as 100 to 4. Better evidence could hardly be desired of the potent influence of a cross with a fresh stock on plants which had been self-fertilised for eight generations, and had been cultivated all the time under nearly uniform conditions, in comparison with plants