The Elements of Bacteriological Technique: A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.Eyre, J. W. H. (John William Henry)
Science
The Elements of Bacteriological Technique: A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.
Eyre, J. W. H. (John William Henry)
Bacteriology -- Technique
The unit of length as applied to the measurement of microscopical
objects is the one-thousandth part of a millimetre (0.001 mm.),
denominated a _micron_ (sometimes, and erroneously, referred to as a
micro-millimetre), and indicated in writing by the Greek letter µ. Of
the many methods in use for the measurement of bacteria, three only will
be here described, viz.:
(a) By means of the Camera Lucida.
(b) By means of the ocular or Eyepiece Micrometer.
(c) By means of the Filar Micrometer (Ramsden's micrometer eyepiece).
For each of these methods a ~stage micrometer~ is necessary. This is a 3
by 1 inch glass slip having engraved on it a scale divided to hundredths
of a millimetre (0.01 mm.), every tenth line being made longer than the
intervening ones, to facilitate counting; and from these engraved lines
the measurement in every case is evaluated. A cover-glass is cemented
over the scale to protect it from injury.
[Illustration: FIG. 55.--Camera lucida, Abbé pattern.]
(a) By means of the Camera Lucida.
1. Attach a camera lucida (of the Wollaston, Beale, or Abbé pattern)
(Fig. 55) to the eyepiece of the microscope.
2. Adjust the micrometer on the stage of the microscope and accurately
focus the divisions.
3. Project the scale of the stage micrometer on to a piece of paper and
with pen or pencil sketch in the magnified image, each division of which
corresponds to 10µ. Mark on the paper the optical combination (ocular
objective and tube length) employed to produce this particular
magnification.
4. Repeat this procedure for each of the possible combinations of
oculars and objectives fitted to the microscope supplied, and carefully
preserve the scales thus obtained.
To measure an object by this method simply project the image on to the
scale corresponding to the particular optical combination in use at the
moment. Read off the number of divisions it occupies and express them as
_micra_.
In place of preserving a scale for each optical combination, the object
to be measured and the micrometer scale may be projected and sketched,
in turn, on the same piece of paper, taking particular care that the
centre of the eyepiece is 25 cm. from the paper on which the divisions
are drawn.
[Illustration: FIG. 56.--Eyepiece micrometer, ordinary.]
[Illustration: FIG. 57.--Eyepiece micrometer, net.]
(b) By means of the Eyepiece Micrometer.
The ~eyepiece micrometer~ is a circular glass disc having engraved on it a
scale divided to tenths of a millimetre (0.1 mm.) (Fig. 56), or the
entire surface ruled in 0.1 mm. squares (the net micrometer) (Fig. 57).
It can be fitted inside the mount of any ocular just above the aperture
of the diaphragm and must be adjusted exactly in the focus of the eye
lens.
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