The Elements of Bacteriological Technique: A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.Eyre, J. W. H. (John William Henry)
Science
The Elements of Bacteriological Technique: A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.
Eyre, J. W. H. (John William Henry)
Bacteriology -- Technique
Some makers mount the glass disc together with a circular cover-glass in
such a way that when placed in position in any Huyghenian eyepiece of
their own manufacture, the scale is exactly in focus for normal vision.
Special eyepieces are also obtainable having a sledging adjustment to
the eye lens for focussing the micrometer.
The value of one division of the micrometer scale must first be
ascertained for each optical combination by the aid of the stage
micrometer, thus:
1. Insert the eyepiece micrometer inside the ocular and adjust the stage
micrometer on the stage of the microscope.
2. Focus the scale of the stage micrometer accurately; the lines will
appear to be immediately below those of the eyepiece micrometer. Make
the lines on the two micrometers parallel by rotating the ocular.
3. Make two of the lines on the ocular micrometer coincide with those
bounding one division of the stage micrometer; this is effected by
increasing or diminishing the tube length; and note the number of
included divisions.
4. Calculate the value of each division of the eyepiece micrometer in
terms of µ, by means of the following formula:
x = 10 y.
Where x = the number of included divisions of the
eyepiece micrometer.
y = the number of included divisions of the
stage micrometer.
5. Note the optical combination employed in this experiment and record
it with the calculated micrometer value.
Repeat this process for each of the other combinations. Carefully record
the results.
To measure an object by this method read off the number of divisions of
the eyepiece micrometer it occupies and express the result in _micra_ by
a reference to the standard value for the particular optical combination
employed.
Zeiss prepares a compensating eyepiece micrometer for use with his
apochromatic objectives, the divisions of which are so computed that
(with a tube length of 160 mm.) the value of each is equivalent to as
many _micra_ as there are millimetres in the focal length of the
objective employed.
_Wright's Eikonometer_ is really a modification of the eyepiece
micrometer for rapidly measuring microscopical objects by direct
inspection, having previously determined the magnifying power of the
particular optical combination employed. It is a small piece of
apparatus resembling an eyepiece, with a sliding eye lens, which can be
accurately focussed on a micrometer scale fixed within the instrument.
When placed over the microscope ocular the divisions of this scale
measure the actual size of the virtual image in millimetres.
In order to use this instrument for direct measurement, it is first
necessary to determine the magnifying power of each combination of
ocular, tube length and objective.
Place a stage micrometer divided into hundredths of a millimetre on the
microscope stage and focus accurately.
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