The Elements of Bacteriological Technique: A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged. — John Shaqi
The Elements of Bacteriological Technique: A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.Eyre, J. W. H. (John William Henry)
Science
The Elements of Bacteriological Technique: A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.
Eyre, J. W. H. (John William Henry)
Bacteriology -- Technique
1. Pipettes of 1 c.c. capacity graduated in 0.1 c.c.
2. Pipettes of 1 c.c. capacity graduated in 0.01 c.c. (Fig. 10, a).
3. Pipettes of 10 c.c. capacity graduated in 0.1 c.c. (Fig. 10, b).
These should be about 30 cm. in length (1 and 2 of fairly narrow bore),
graduated to the extreme point, and having at least a 10 cm. length of
clear space between the first graduation and the upper end; the open
mouth should be plugged with cotton-wool. Each variety should be
sterilised and stored in a separate cylindrical copper case some 36 by 6
cm., with "pull-off" lid, upon which is stamped, in plain figures, the
capacity of the contained pipettes.
[Illustration: FIG. 10.--Measuring pipettes, a and b.]
The laboratory should also be provided with a complete set of "Standard"
graduated pipettes, each pipette in the set being stamped and
authenticated by a certificate from one of the recognised Physical
Measurement Laboratories, such as Charlottenburg. These instruments are
expensive and should be reserved solely for standardising the pipettes
in ordinary use, and for calibrating small pipettes manufactured in the
laboratory. Such a set should comprise, at least, pipettes delivering 10
c.c., 5 c.c., 2.5 c.c., 2 c.c., 1 c.c., 0.5 c.c., 0.25 c.c., 0.2 c.c.,
0.1 c.c., 0.05 c.c., and 0.01 c.c., respectively.
In the immediately following sections are described small pieces of
glass apparatus which should be prepared in the laboratory from glass
tubing of various sizes. In their preparation three articles are
essential; first a three-square hard-steel file or preferably a
glass-worker's knife of hard Thuringian steel for cutting glass tubes
etc.; next a blowpipe flame, for although much can be done with the
ordinary Bunsen burner, a blowpipe flame makes for rapid work; and
lastly a bat's-wing burner.
[Illustration: FIG. 11.--Glass-cutting knife. a. handle. b. double
edged blade. c. shaft. d. locking nut. e. spanner for nut.]
1. The glass-cutting knife. This article is sold in two forms, a bench
knife (Fig. 11) and a pocket knife. The former is provided with a blade
some 8 cm. in length and having two cutting edges. The cutting edge when
examined in a strong light is seen to be composed of small closely set
teeth, similar to those in a saw. The knife should be kept sharp by
frequent stroppings on a sandstone hone. The pocket form, about 6-cm.
long over all, consists of a small spring blade with one cutting edge
mounted in scales like an ordinary pocket knife.
2. For real convenience of work the blowpipe should be mounted on a
special table connected up with cylindrical bellows operated by a pedal.
That figured (Fig. 12) is made by mounting a teak top 60 cm. square upon
the uprights of an enclosed double-action concertina bellows (Enfer's)
and provided with a Fletcher's Universal gas blowpipe.
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