The Elements of Bacteriological Technique: A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.Eyre, J. W. H. (John William Henry)
Science
The Elements of Bacteriological Technique: A Laboratory Guide for Medical, Dental, and Technical Students. Second Edition Rewritten and Enlarged.
Eyre, J. W. H. (John William Henry)
Bacteriology -- Technique
3. An ordinary bat's-wing gas-burner mounted at the far corner of the
table top is invaluable in the preparation of tubular apparatus with
sharp curves, and for coating newly-made glass apparatus with a layer of
soot to prevent too rapid cooling, and its usually associated
result--cracking.
[Illustration: FIG. 12.--Glass blower's table with Enfer's foot
bellows.]
6. ~Sedimentation tubes 5×0.5~ cm., for sedimentation reactions, etc., and
for containing small quantities of fluid to be centrifugalised in the
hæmatocrit. These are made by taking 14-cm. lengths of stout glass
tubing of the requisite diameter and heating the centre in the Bunsen or
blowpipe flame. When the central portion is quite soft draw the ends
quickly apart and then round off the pointed ends of the two test-tubes
thus formed. With the glass-cutting knife cut off whatever may be
necessary from the open ends to make the tubes the required length.
A rectangular block of "plasticine" (modelling clay) into which the
conical ends can be thrust makes a very convenient stand for these small
tubes.
~Capillary Pipettes or Pasteur's Pipettes~ (Fig. 13 a).--These little
instruments are invaluable, and a goodly supply should be kept on hand.
They are prepared from soft-glass tubing of various-sized calibre (the
most generally useful size being 8 mm. diameter) in the following
manner: Hold a 10 cm. length of glass tube by each end, and whilst
rotating it heat the central portion in the Bunsen flame or the blowpipe
blast-flame until the glass is red hot and soft. Now remove it from the
flame and steadily pull the ends apart, so drawing the heated portion
out into a roomy capillary tube; break the capillary portion at its
centre, seal the broken ends in the flame, and round off the edges of
the open end of each pipette. A loose plug of cotton-wool in the open
mouth completes the capillary pipette. After a number have been
prepared, they are sterilised and stored in batches, either in metal
cases similar to those used for the graduated pipettes or in large-sized
test-tubes--sealed ends downward and plugged ends toward the mouth of
the case.
[Illustration: FIG. 13.--Capillary pipettes. a, b, c.]
The filling and emptying of the capillary pipette is most satisfactorily
accomplished by slipping a small rubber teat (similar to that on a
baby's feeding bottle but _not perforated_) on the upper end, after
cutting or snapping off the sealed point of the capillary portion. If
pressure is now exerted upon the elastic bulb by a finger and thumb
whilst the capillary end is below the surface of the fluid to be taken
up, some of the contained air will be driven out, and subsequent
relaxation of that pressure (resulting in the formation of a partial
vacuum) will cause the fluid to ascend the capillary tube. Subsequent
compression of the bulb will naturally result in the complete expulsion
of the fluid from the pipette (Fig. 14).
[Illustration: FIG. 14.--Filling the capillary teat-pipette.]
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