The transformation from the telophase of the last spermatogonial division to the prophase of the first spermatocyte is marked by practically the same changes in both families. It is to be observed, however, that the derivation of the spireme from the disintegrating chromosomes of the previous generation is not so clearly indicated in the Locustid cells, and it was for this reason that in the examination of _Xiphidium_ I was not able to determine certainly that the accessory chromosome came over from the spermatogonia into the spermatocytes as a formed element. Upon this point, as upon others, my later material is clearer, and I was able to reconcile the appearances in the two families. In both, unfortunately, it has been found impossible to determine the exact origin of the first spermatocyte chromosomes. In connection with the transformation of the chromatin from the spermatogonial condition to that of the spermatocyte, we must take notice of that stage which is commonly denominated the “synapsis.” The evidence afforded by the Orthopteran cells is entirely negative regarding this. In properly fixed material there is no distortion of the chromatin in the nucleus at any time. It would, if present, be particularly easy to observe, as was stated in my previous paper, for during the entire winter the spermatocytes exist in the spireme stage, and in a longitudinal section of a follicle all stages may be discerned. On the other hand, in poorly fixed or hastily prepared material the synapsis is present, and always in such a form as to indicate its artificial character. What is here said regarding the synapsis refer to the appearance commonly thus designated, but, as has already been stated, such an application of the term does not meet the spirit of the definition as intended by Moore (=20=). A fusion of the spermatogonial chromosomes of some sort must certainly occur, but that it is always marked by a unilateral massing of chromosomes, I deny.