Pasteur, Louis, 1822-1895; Scientists -- France -- Biography
Three hens were lying on the table, all of them dead. The first one had
been inoculated under the thorax with five drops of yeast water slightly
alkalized, which had been given as a nutritive medium to some bacteridia
anthracis; the hen had been placed in a bath at 25° C., and had died
within twenty-two hours. The second one, inoculated with ten drops of a
culture liquid, had been placed in a warmer bath, 30° C., and had died
in thirty-six hours. The third hen, also inoculated and immersed, had
died in forty-six hours.
Besides those three dead hens, there was a living one which had been
inoculated in the same way as the first hen. This one had remained for
forty-three hours with one-third of its body immersed in a barrel of
water. When it was seen in the laboratory that its temperature had gone
down to 36° C., that it was incapable of eating and seemed very ill, it
was taken out of the tub that very Saturday morning, and warmed in a
stove at 42° C. It was now getting better, though still weak, and gave
signs of an excellent appetite before leaving the Academy council
chamber.
The third hen, which had been inoculated with ten drops, was dissected
then and there. Bouley, after noting a serous infiltration at the
inoculation focus, showed to the judges sitting in this room, thus
suddenly turned into a testing laboratory, numerous bacteridia scattered
throughout every part of the hen.
“After those ascertained results,” wrote Bouley, who drew up the report,
“M. Colin declared that it was useless to proceed to the necropsy of the
two other hens, that which had just been made leaving no doubt of the
presence of bacilli anthracis in the blood of a hen inoculated with
charbon and then placed under the conditions designated by M. Pasteur as
making inoculation efficacious.
“The hen No. 2 has been given up to M. Colin to be used for any
examination or experiment which he might like to try at Alfort.
“Signed: G. Colin, H. Bouley, C. Davaine, L. Pasteur, A. Vulpian.”
“This is a precious autograph, headed as it is by M. Colin’s signature!”
gaily said Bouley. But Pasteur, pleased as he was with this conclusion,
which put an end to all discussion on that particular point, was already
turning his thoughts into another channel. The Academician who had
joined the members of the Commission was showing him a number of the
_Revue Scientifique_ which had appeared that morning, and which
contained an article of much interest to Pasteur.
In October, 1877, Claude Bernard, staying for the last time at St.
Julien, near Villefranche, had begun some experiments on fermentations.
He had continued them on his return to Paris, alone, in the study which
was above his laboratory at the Collège de France.
Public-domain text, read in full here on John Shaqi.
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