The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science — John Shaqi
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
Further investigations and experiments led Abbe to discard so much of
his theoretical conclusions relating to superimposed images having
a distinct character as well as a different origin, and as to their
capability of being separated and examined apart from each other.
In a later paper he writes: “I no longer maintain in principle the
distinction between the absorption image or direct dioptrical image and
the diffraction image, nor do I hold that the microscopical image of
an object consists of two superimposed images of different origin or a
different mode of production. Thus it appears that both the absorption
image and the diffraction image he held to be equally of diffraction
origin; but while a lens of small aperture would give the former with
facility, it would be powerless to reveal the latter, because of its
limited capacity to gather in the strongly-deflected rays due to the
excessively minute bodies the microscopical objective has to deal
with.”[11]
Abbe’s theory of vision has been questioned by mathematicians, and
since his death Lord Rayleigh went more deeply into the question of
“the theory of the formation of optical images,” with special reference
to the microscope and telescope. He has shown that two lines cannot
be fairly resolved unless their components subtend an angle exceeding
that subtended by the wave-length of light at a distance equal to the
aperture; also, that the measure of resolution is only possible with
a square aperture, or one bounded by straight lines, parallel to the
lines resolved.
Lord Rayleigh’s Theory of the Formation of Optical Images, with Special
Reference to the Microscope.[12]
Of the two methods adopted, that of Helmholtz’s consists in tracing
the image representative of a mathematical point in the object, the
point being regarded as self-luminous; that of Abbe’s the typical
object was not, as we have seen, a luminous _point_, but a _grating_
illuminated by plane waves of light. In the latter method, Lord
Rayleigh argues that the complete representation of the object
requires the co-operation of all the spectra which are focussed in the
principal focal plane of the objective; when only a few are present
the representation is imperfect, and wholly fails when there is only
one. He then proceeds to show, by the aid of diagrams and mathematical
formula, how the resolving power can be adduced.
Public-domain text, read in full here on John Shaqi.
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