The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
On further criticism of the Abbe spectrum theory, he observes “that
although the image ultimately formed may be considered to be due to
the spectra focussed to a given point, the degree of conformity of
the image to the object is another question. The consideration of the
case of a very fine grating, which might afford no lateral spectra at
all, shows the incorrectness of the usually accepted idea that if all
the spectra are utilised the image will still be incomplete, so that
the theory (originally promulgated by Abbe) requires a good deal of
supplementing; while it is inapplicable when the incident light is
not parallel, and when the object is, for example, a double point and
not a grating. Even in the case of a grating, the spectrum theory is
inapplicable, if the grating is self-luminous; for in this case no
spectra can be formed since the radiations from the different elements
of the grating have no permanent phase-relations.” For these reasons
Lord Rayleigh advises that the question should be reconsidered from
the older point of view, according to which the typical object is a
point and not a grating. Such treatment will show that the theory of
resolving power is essentially the same for all instruments. The
peculiarities of the microscope, arising from the divergence-angles
not being limited to be small, and from the different character of
the illumination, are theoretically only differences of detail. These
investigations can be extended to gratings, and the results so obtained
confirm for the most part the conclusions of the spectrum theory.
Furthermore, that the function of the condenser in microscopic practice
in throwing upon the object the image of the lamp-flame is to cause
the object to behave, at any rate in some degree, as if it were
self-luminous, and thus to obviate the sharply-marked interference
bands which arise when permanent and definite phase-relations are
permitted to exist between the radiations which issue from various
points of the object. This is capable of mathematical proof; and in
the case where the illumination is such that each point of the row or
of the grating radiates independently, the limit to resolution is seen
to depend only on the width of the aperture, and thus to be the same
for all forms of aperture as for those of the rectangular. That Abbe’s
theory of microscopic vision is fairly open to the criticisms passed on
it by Lord Rayleigh must be taken for granted.
Definition of Aperture; Principles of Microscopic Vision.
Public-domain text, read in full here on John Shaqi.
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