The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
Fig. 35.--Relative diameters of the (utilized) back lenses of various
dry and immersion objectives of the same power (1/4-in.) from an air
angle of 60° to an oil angle of 180°.]
Thus we arrive at a general proposition for all kinds of objectives:
1st, when the power is the same, the admission of rays (or aperture)
varies with the diameter of the pencil at its emergence; 2nd, when the
powers are different, the same aperture requires different openings in
the ratio of the focal lengths, or conversely with the same opening the
aperture is in inverse ratio to the focal lengths. We see, therefore,
that just as in the telescope the absolute diameter of the object-glass
defines its _aperture_, so in the microscope _the ratio between the
utilised diameter of the back lens and the focal length_ of the
objective defines its aperture also, and this is clearly a definition
of aperture in its primary and only legitimate meaning as “opening;”
that is, the capacity of the objective for admitting rays from the
object and transmitting them to the image.
If, by way of illustration, we compare a series of dry and
oil-immersion objectives, and commencing with small air angles,
progress up to 180° air angle, and then take an oil-immersion of 82°
and progress again to 180° oil angle, the ratio of opening to power
progresses also, and attains its maximum, not in the case of the air
angle of 180° (when it is exactly equivalent to the oil angle of only
82°), but is greatest at the oil angle of 180°. If we assume the
objectives to have the same power throughout we get rid of one of the
factors of the ratio, and we have only to compare the diameters of the
emergent beams, and can represent their relations by diagrams.
Fig. 35 illustrates five cases of different apertures of 1/4-in.
objectives, viz.: those of dry objectives of 60°, 97°, and 180° air
angle, a water-immersion of 180° water angle, and an oil-immersion of
180° oil angle. The inner dotted circles in the two latter cases are of
the same size as that corresponding to the 180° air angle.
A dry objective of the maximum air angle of 180° is only able to
utilise a diameter of back lens equal to twice the focal length, while
an immersion lens of even only 100° utilises a _larger_ diameter,
_i.e._, it is able to transmit more rays from the object to the image
than any dry objective is capable of transmitting. Whenever the angle
of an immersion lens exceeds twice the critical angle for the immersion
fluid, _i.e._, 96° for water or 82° for oil, its aperture is in excess
of that of a dry objective of 180°.
[Illustration: Fig. 36.]
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