The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
24. We will now consider the best methods of _mounting_ injected
objects. They must always be well washed in water after they have been
kept in any preservative liquid, using a camel-hair pencil to clean the
surface if necessary. Many parts when injected are in masses, such as
the lungs, liver, &c., of animals, and consequently sections of these
must be cut. For this purpose Valentine’s knife is very convenient,
as the thickness can be regulated so easily; but where the injections
are opaque, there is no need to have the sections very thin. Some few
of this kind undergo comparatively little change in drying, so that
the section may be well washed and floated upon the glass slide in the
place desired, where it will dry perfectly and adhere to it. It must
be then moistened with turpentine and mounted in Canada balsam like
other objects. No great heat should be used with these preparations,
as it is very liable to injure them; and some of the colours seem to
suffer a slight contraction when any great degree of warmth is applied.
There are many objects, however, which must be seen in the mass to be
understood, and, indeed, lose all their form and beauty in drying, such
as certain parts of the intestines, &c. These must be mounted in fluid,
with the precautions noticed at length in Chapter IV., and for this
purpose either Goadby’s fluid, the chloride of zinc solution, or spirit
diluted with ten parts of distilled water, may be employed. It is a
good thing, when practicable, to mount similar objects on two separate
slides, using different preservative liquids, and taking the precaution
of marking each with the kind of liquid employed. This not only serves
as a guide to what is best for certain subjects, but if one is injured,
there will probably be a good specimen in the other.
25. It may be here mentioned that many are now mounting sections of
injected substances with the balsam and chloroform before mentioned,
instead of using balsam alone, and consider that the labour is much
lessened thereby.
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