The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
In applying the required heat to the slides, covers, &c., it is
necessary in many cases to ensure uniformity, otherwise there is danger
of the glass being broken. For this purpose a brass plate at least
three inches wide, somewhat longer, and one-eighth of an inch thick,
must be procured. It should then be affixed to a stand, so that it may
be readily moved higher or lower, in order that the distance from the
lamp may be changed at will, and thus the degree of heat more easily
regulated. This has also the advantage of enabling the operator to
allow his slides, &c., to cool more gradually, which, in some cases, is
absolutely necessary,--as in fusing some of the salts, &c.
In order to get rid of air-bubbles, which are frequently disagreeable
enemies to the mounter of objects, an air-pump is often very useful.
This is made by covering a circular plate of metal with a bell-glass,
both of which are ground so finely at the edges that greasing the place
of contact renders it air-tight. The pump is then joined to the metal
plate underneath, and worked with a small handle like a common syringe.
By turning a small milled head the air may be allowed to re-enter when
it is required to remove the bell-glass and examine or perform any
operation upon the object. The mode of using this instrument will be
described hereafter, but it may be here stated that substitutes have
been devised for this useful apparatus; but as it is now to be obtained
at a low cost, it is hardly worth while to consider them. Much time is,
in many instances, certainly saved by its use, as a very long immersion
in the liquids would be required to expel the bubbles, where the
air-pump would remove them in an hour.
The next thing to be considered is what may be termed CEMENTS, some
of which are necessary in every method of mounting objects for the
microscope. Of these will be given the composition where it is
probable the young student can make use of it; but many of them are
so universally kept as to be obtainable almost anywhere; and when
small quantities only are required, economy suffers more from home
manufacture than from paying the maker’s profit.
Public-domain text, read in full here on John Shaqi.
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