The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
Again, some objects when placed upon the glass slide are of such an
elastic nature that no cement will secure the thin glass covering until
it becomes hard. This difficulty may be overcome by various methods.
The following are as good and simple as any. Take two pieces of wood
about two inches long, three-quarters wide, and one-quarter thick;
and a small rounded piece one inch long and one-quarter in diameter;
place this latter betwixt the two larger pieces. Over one end of the
two combined pass an india-rubber band. This will give a continual
pressure, and may be opened by bringing the two pieces together at
the other end; the pressure may be readily made uniform by paring the
points at the inner sides, and may be regulated by the strength of the
india-rubber band. These bands may be made cheaply, and of any power,
by procuring a piece of india-rubber tubing of the width required, and
cutting off certain breadths. Another very simple method of getting
this pressure is mentioned in the “Micrographic Dictionary.” Two pieces
of whalebone of the length required are tied together firmly at each
end. It is evident that any object placed betwixt them will be subject
to continual pressure. The power of this may be regulated by the
thickness and length of the whalebone. This simple contrivance is very
useful.
Common watch-glasses should always be kept at hand. They are certainly
the cheapest, and their transparency makes them very convenient
reservoirs in which objects may be steeped in any liquid; as it
saves much trouble to examine cursorily under the microscope, when
the air-bubbles are expelled from insects, &c., &c. They are readily
cleaned, and serve very well as covers, when turned upside down,
to protect any objects from the dust. For this latter purpose Dr.
Carpenter recommends the use of a number of bell-glasses, especially
when one object must be left for a time (which often happens) in order
that another may be proceeded with. Wine glasses, when the “legs” are
broken, may thus be rendered very useful.
As heat is necessary in mounting many objects, a lamp will be required.
Where gas is used, the small lamp known as “Bunsen’s” is the most
convenient and inexpensive. It gives great heat, is free from smoke,
and is readily affixed to the common gas-burner by a few feet of
india-rubber tubing. The _light_ from these lamps is small, but this is
little or no drawback to their use. Where gas is not available, the
common spirit-lamps may be used, which are very clean and answer every
purpose.
Public-domain text, read in full here on John Shaqi.
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