The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
It must be used whilst hot, and as long as it retains even slight
warmth can be readily moulded into any form. It is often employed in
making shallow cells for liquids, as before mentioned.
GUM-WATER is an article which nobody should ever be without; but
labels, or indeed any substance, affixed to glass with common gum, are
so liable to leave it spontaneously, especially when kept very dry,
that I have lately added five or six drops of glycerine to an ounce of
the gum solution. This addition has rendered it very trustworthy even
on glass, and now I never use it without. This solution cannot be kept
long without undergoing fermentation, to prevent which the addition
of a small quantity of any essential oil (as oil of cloves, &c.),
or one-fourth of its volume of alcohol, may be made, which will not
interfere in any way with its use.
There is what is sometimes termed an _extra adhesive_ gum-water, which
is made with the addition of isinglass, thus:--Dissolve two drachms of
isinglass in four ounces of distilled vinegar; add as much gum arabic
as will give it the required consistency. This will keep very well, but
is apt to become thinner, when a little more gum may be added.
All these, except one or two, are liquid, and must be kept in
_stoppered_ bottles, or, at least, as free from the action of the air
as possible.
When any two substances are to be united firmly, I have termed the
medium employed “a cement;” but often the appearance of the slides is
thought to be improved by drawing a coloured ring upon them, extending
partly on the cover and partly on the slide, hiding the junction of the
two. The medium used in these cases I term A VARNISH, and hereinafter
mention one or two. Of course, the tenacity is not required to be so
perfect as in the _cements_.
SEALING-WAX VARNISH is prepared by coarsely powdering sealing wax,
and adding spirits of wine; it is then digested at a gentle heat to
the required thickness. This is very frequently used to “finish” the
slides, as before mentioned, and can easily be made of any colour by
employing different kinds of sealing-wax; but is very liable to “chip”
and leave the glass.
BLACK VARNISH is readily prepared by adding a small quantity of
lamp-black to gold-size and mixing intimately. Dr. Carpenter recommends
this as a good finishing varnish, drying quickly and being free from
that brittleness which renders some of the others almost worthless; but
it should not be used in the first process when mounting objects in
fluid.
Amongst these different cements and varnishes I worked a long time
without coming to any decision as to their comparative qualities,
though making innumerable experiments. The harder kinds were
continually cracking, and the softer possessed but little adhesive
power. To find hardness and adhesiveness united was my object, and the
following possesses these qualities in a great degree:--
India-rubber ½ drachm.
Asphaltum 4 oz.
Mineral naphtha 10 ”
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