The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
When gum or other liquid cement has been used to fix the objects to
the glass, the thin covers must not be applied until the slide has
been _thoroughly dried_, and all fear of dampness arising from the use
of the solution done away with. Warmth may be safely applied for the
purpose, as objects fastened by this method are seldom, if ever, found
to be loosened by it. As objects are met with of every thickness, the
cells will be required of different depths. There is no difficulty in
accommodating ourselves in this--the deeper cells may be readily cut
out of thick leather, card, or other substance preferred (as mentioned
in Chapter I.). Cardboard is easily procured of almost any thickness;
but sometimes it is convenient to find a thinner substance even than
this. When thin glass is laid upon a drop of any liquid upon a slide,
every one must have observed how readily the liquid spreads betwixt
the two: just so when any thin varnish is used to surround an object
of little substance, excessive care is needed lest the varnish should
extend betwixt the cover and slide, and so render it worthless. The
slightest wall, however, prevents this from taking place, so that a
ring of common paper may be used, and serve a double purpose where the
objects require no deeper cell than this forms.
Many objects, however, are of such tenuity--as the leaves of many
mosses, some of the Diatomaceæ, scales of insects, &c.--that no cell is
requisite excepting that which is necessarily formed by the medium used
to attach the thin glass cover to the slide; and where the slide is
covered by the ornamental papers mentioned in Chapter I., and pressure
does not injure the object, even this is omitted, the thin glass being
kept in position by the cover; but slides mounted in this manner are
frequently injured by dampness, which soon condenses upon the inner
surfaces and interferes both with the object and the clearness of its
appearance.
The thin glass, then, is to be united to the slide, so as to form a
perfect protection from dust, dampness, or other injurious matter,
and yet allow a thoroughly distinct view of the object. This is to be
done by applying to the glass slide round the object some adhesive
substance, and with the forceps placing the thin glass cover (quite dry
and clean) upon it. A gentle pressure round the edge will then ensure
a perfect adhesion, and with ordinary care there will be little or no
danger of breakage. For this purpose gold-size is frequently used. The
asphalt and india-rubber varnish also will be found both durable and
serviceable. Whatever cement may be used, it is well to allow it to
become in some measure “fixed” and dried; but where no cell or “wall”
is upon the slide, this is _quite necessary_, otherwise the varnish
will be almost certain to extend, as before mentioned, and ruin the
object. It may be stated here that gold-size differs greatly in its
drying powers, according to its age, mode of preparation, &c. (Chapter
IV.)
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