The preparation & mounting of microscopic objects — John Shaqi
The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
When mounting an object in any of these cells, the glass must be
thoroughly cleaned, which may be done with a cambric handkerchief,
after the washing mentioned in Chapter I. _If the object be large_,
the point of a fine camel-hair pencil should be dipped into the gum
solution, and a minute quantity of the liquid deposited in the cell
where the object is to be placed, but not to cover a greater surface
than the object will totally hide from sight. This drop of gum must be
allowed to dry, which will take a few minutes. Breathe then upon it two
or three times, holding the slide not far from the mouth, which will
render the surface adhesive. Then draw a camel-hair pencil through the
lips, so as to moisten it slightly (when anything small will adhere to
it quite firmly enough), touch the object and place it upon the gum in
the desired position. This must be done immediately to ensure perfect
stability, otherwise the gum will become at least partially dry and
only retain the object imperfectly.
When, however, the objects are so minute that it would be impossible to
deposit atoms of gum small enough for each one to cover, a different
method of proceeding must be adopted. In this case a small portion of
the same gum solution should be placed upon the slide, and by means
of any small instrument--a long needle will serve the purpose very
well--spread over the surface which will be required. The quantity thus
extended will be very small, but by breathing upon it may be prevented
drying whilst being dispersed. This, like the forementioned, should be
then allowed to dry; and whilst the objects are being placed on the
prepared surface, breathing upon it as before will restore the power of
adherence.
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