The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
It has been stated in another place that it is assumed the operator is
not mounting diatoms simply as microscopic objects, but as instructive
specimens. It is not, therefore, sufficient to take a single slide as
all that is required, but to have the same diatom prepared in as many
ways as possible. The following are the principal:--
1. Mounted crude in fluid (see Chapter IV.).
2. Burnt crude upon the cover, and mounted dry or in balsam (as
before mentioned).
3. Mounted dry or in balsam (see Chapter III.), after the
cleansing process already described.
I will here give Mr. Rylands’ method of mounting them _dry_, the fluid
and balsam preparations being noticed in their respective chapters.
The slide with the ring of asphalt, or black varnish, should have been
prepared some weeks previously, in order to allow it to dry thoroughly.
When required, it must be held over the spirit-lamp or Bunsen’s burner
until the ring of varnish is softened. The burnt cover, having been
heated at the same time, must then be taken in the forceps and pressed
upon the softened varnish until it adheres all round. When cold, an
outer ring of asphalt completes the slide.
Such is the method which my friend Mr. T. G. Rylands employs in
the preparation of diatoms for the microscope. I have said enough
concerning his results. It is to be feared, however, that to some these
several modes of operation may appear lengthy and complicated; but if
read carefully, and the experiments tried, they will be found simple
enough in practice, and to occupy much less time than an intelligible
description would lead the novice to believe necessary.
Public-domain text, read in full here on John Shaqi.
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