The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
We will now consider the mode of mounting the prepared diatoms, which,
if used dry (as described in this chapter), should be carefully washed
two or three times with the purest distilled water. In this branch,
as in every other, each collector gives preference to that method in
which he is an adept. Thus the diatoms may be placed on the under side
of the cover, to be as near to the object-glass as possible, or upon
the slide itself; and each plan has its advocates. Whichsoever of these
is used, nothing seems more simple to the novice than a tolerably
equal dispersion of the objects upon the slide or cover; but this is
by no means so readily accomplished, consequently I give Mr. Rylands’
method, as his slides are perfect in this respect also. He always
places the diatoms upon the thin glass cover. It is not sufficient,
as is frequently thought, to take a drop of liquid containing the
cleansed material and spread it upon the cover or slide, as without
some additional precaution that uniform and regular distribution of the
specimens is not obtained which is desirable. In order to effect this,
let a drop of the cleansed gathering be diluted sufficiently for the
purpose--how much must be determined in each case by experiment--and
let the covers to be mounted be cleaned and laid upon the brass plate.
(See Chapter I.) By means of a glass tube, about one-twelfth of an
inch in diameter, stopped by the wetted finger at the upper end, take
up as much of the diluted material as will form a moderately convex
drop extending over the whole cover. When all the covers required are
thus prepared, apply a lamp below the brass plate, and raise the
temperature to a point just short of boiling. By this means the covers
will be dried in a few minutes, and the specimens equally distributed
over the whole area. The spread of the fluid upon the covers is
facilitated by breathing upon them; and, to insure uniformity, care
must be taken to avoid shaking them whilst drying. The best plan is to
mount at least half a dozen at once.
Before mounting, Mr. Rylands always burns the diatoms upon the glass
at a dull red heat, whether they are used with balsam or dry. This
burning, he says, is not only an additional cleaning process, but
it effectually fixes the diatoms, and prevents them floating out if
mounted with balsam. The thinnest covers may be burnt without damage
if they are placed upon a small piece of platinum foil of the size
required, which should be about one-hundredth of an inch thick,
perfectly flat, and having three of its edges slightly bent over, so as
to prevent its warping with the heat. The small flame of a spirit-lamp,
or, where there is gas, a Bunsen’s burner, may be employed. The cover
should be shaded from direct daylight, that the action of the flame
may be observed more perfectly. Care must then be taken to raise the
temperature only to the dull red heat before mentioned. The cover will
then be in a fit state for mounting as required.
Public-domain text, read in full here on John Shaqi.
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