The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
On the surface of the leaves, hairs and scales of various and very
beautiful forms are found, most of which display their beauties best
when removed from the leaf, and used with the polarizer. These will be
noticed in another place; but a portion of the leaf should always be
prepared in its natural form, to show the arrangement of the hair or
scales upon it; which must almost invariably be mounted dry when used
for this purpose. Many of them require very delicate handling. The
_epidermis_, or, as it is by some termed, the _cuticle_, is the outer
skin which lies upon the surface of the leaves and other parts of most
plants. This is composed of cells closely connected, often bearing
the appearance of a rude network. In many plants, by scraping up the
surface of the leaf, a thin coating is detached, which may be torn off
by taking hold of it with the forceps. The piece may then be washed and
floated upon a glass slide, where, on drying, it will be firmly fixed,
and may usually be mounted dry. Amongst the most beautiful and easily
prepared of these may be mentioned the petal of the geranium, the cells
of which are well defined and amongst the most interesting.
Closely connected with the leaves are the ANTHERS and POLLEN, of
which a great number are beautiful and interesting subjects for the
microscopist.
The mallow tribe will furnish some exquisite objects, bearing the
appearance of masses of costly jewels. These are usually dried with
pressure, but the natural form may be more accurately preserved by
allowing them to dry as they are taken from the flower, with no
interference except thoroughly protecting them from all dust. Sometimes
the anther is divided, so that the cell required to receive them may
be of as little depth as possible. The common mallow is a beautiful
object, but I think the lavatera is a better, as it shows the pollen
chambers well, when dried unpressed. The pollen is often set alone, and
is well worth the trouble, as it then admits of more close examination.
Often it is convenient to have the _anther and pollen_ as seen in
nature on one slide, and the _pollen_ alone upon another. The former
should be taken from the flowers before their full development is
attained, as if overgrown they lose much of their beauty. Some pollens
are naturally so dark that it is necessary to mount them in Canada
balsam or fluid, as described in other places; but they are better
mounted dry when they are not too opaque.
Here, too, we may also mention the SEEDS of many plants as most
interesting, and some of them very beautiful, objects, requiring for
the greater part but a low power to show them. Most of these are to be
mounted dry, as opaque objects, in cells suited to them, but some are
best seen in balsam, and will be mentioned in Chapter III.
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