The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
Sometimes slides are used which are made by taking a thin slip of wood
of the usual size (3 in. by 1 in.), in the centre of which is cut a
circular hole large enough to receive the object. A piece of thin
glass is fixed underneath the slide, forming a cell for the object,
which may then be covered and finished like an ordinary slide. This
has the advantage of serving for transparent objects for which the
before-mentioned wooden slides are unsuitable. A slight modification
of this plan is often used where the thickness of the objects is
inconsiderable, especially with some of the Diatomaceæ, often termed
“test-objects.” The wooden slide is cut with the central opening as
above, and two pieces of thin glass are laid upon it, betwixt which the
diatoms or other objects are placed, and kept in their proper position
by a paper cover. This arrangement is a good one, insomuch as the very
small portion of glass through which the light passes on its way to the
microscope from the reflector causes the refraction or interference to
be reduced to the lowest point.
A novice would naturally think the appearance of some of the slides
above mentioned very slovenly and unfinished; but they are often
covered with ornamental papers, which may be procured at almost every
optician’s, at a cost little more than nominal, and of innumerable
patterns and colours. How to use these will be described in another
place.
It is very probable that a beginner would ask his friend what kind of
slides he would advise him to use. Almost all those made of wood are
liable to warp more or less, even when the two pieces are separate or
of different kinds; those of cardboard and wood are generally free
from this fault, yet the slides, being opaque, prevent the employment
of the Lieberkuhn. To some extent glass slides, when covered with
ornamental papers, are liable to the same objection, as the light is
partly hindered. And sometimes the dampness from the paste, or other
substance used to affix the papers, penetrates to the object, and so
spoils it, though this may be rendered less frequent by first attaching
the _thin_ glass to the slide by some harder cement. Much time,
however, is taken up by the labour of covering the slides, which is a
matter of consideration with some. Certainly, the cost of the glass
slides was formerly great; but now they are reasonable enough in this
respect, so that this objection is removed. It is, therefore, well to
use glass slides, except where the thin glasses are employed for tests,
&c., as above. When the thin glass circles are placed upon the slides,
and the edge is varnished with black or coloured rings, the appearance
of finish is perfect. The trouble is much less than with most of
the other methods, and the illumination of the object very slightly
interfered with.
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