The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
To varnish the edges of these covers, make circles of any liquid upon
the glass slide, and perform any other “circular” work mentioned
hereafter, the little instrument known as “Shadbolt’s turntable” is
almost indispensable. It is made as follows:--At one end of a small
piece of hard wood is fixed an iron pivot about one-eighth inch thick,
projecting half an inch from the wood, which serves as a centre upon
which a round brass table three inches in diameter revolves. On the
surface of this are two springs, about one and a half inches apart,
under which the slide is forced and so kept in position, whilst the
central part is left open to be worked upon. The centre is marked, and
two circles half an inch and one inch in diameter are usually deeply
engraved upon the table to serve as guides in placing the slide, that
the ring may be drawn in the right position. When the slide is placed
upon the “table” underneath the springs, a camel-hair pencil is filled
with the varnish, or other medium used, and applied to the surface
of the glass; the table is then made to revolve, and a circle is
consequently produced, the diameter of which it is easy to regulate.
Many objects for the microscope may be seriously injured by allowing
the fingers to touch them--many more are so minute that they cannot
be removed in this way at all, and often it is necessary to take
from a mass of small grains, as in sand, some particular particle.
To accomplish this, there are two or three contrivances recommended:
one by means of split bristles, many of which will readily be found
in any shaving brush when it has been well used. The bristles when
pressed upon any hard surface open, and when the pressure is removed
close again with a spring; but the use of these is limited. Camel-hair
pencils are of great service for this and many other purposes to the
microscopist. In _very_ fine work they are sometimes required so small
that all the hairs with the exception of one or two finer pointed ones
are removed. A few of various sizes should always be kept on hand.
Equally necessary are fine pointed needles. They are very readily put
up for use by thrusting the “eye” end into a common penholder, so as to
be firm. The points may be readily renewed, when injured, on a common
whetstone; but when out of use they may be protected by being thrust
into a piece of cork.
Knives of various kinds are required in some branches of microscopic
work, but these will be described where “dissection,” &c., is treated
at some length, as also various forms of scissors. In the most simple
objects, however, scissors of the usual kind are necessary. Two or
three sizes should always be kept at hand, sharp and in good order.
Public-domain text, read in full here on John Shaqi.
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