The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
the _raphides_ caustic potash may be employed; but after _any_ of these
agents have been made use of, the objects must be thoroughly cleansed
with water, else the dissecting instruments (and perhaps the cell) will
be injured by the action of the remaining portion of the softening
agent.
For the dissection of _animal tissues_ it is necessary that the
instruments be in the best order as to sharpness, &c.; and as the rules
to be observed must necessarily be somewhat alike in many instances,
the treatment required by some of the objects most frequently mounted
will now be described. We may here remark that _cartilage_ can be best
examined by taking sections, which will show the arrangement of the
cells very perfectly. This, however, is plainly seen in the mouse’s
ear without any section being necessary. Glycerine, the preservative
liquids before mentioned, and Canada balsam are all used to mount it;
but perhaps the first named may be preferred in many cases.
MUSCLE.--This is what is commonly called the “flesh” of animals. If a
piece be laid upon the slide under the microscope, bundles of “fibres”
will be perceived, which with needles and a little patience may be
separated into portions, some of these being “striated,” or marked
with alternate spaces of dark and light. Some of the _non-striated_
or _smooth_ class of muscle, such as is found in intestines, may be
prepared for the microscope by immersing for a day or two in nitric
acid diluted with three or four parts of water, and then separating
with needles and mounting as soon as possible. Sometimes _boiling_ is
resorted to to facilitate the separation, and occasions little or no
alteration in the material. Specimens are often taken from the _frog_
and the _pig_, as being amongst the best, _Goadby’s solution_ being
generally used in mounting them. The muscle of insects also shows the
striæ very perfectly.
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