The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
these are generally a quantity of minute hairs, forming a guard against
the entrance of dust, &c. The forms of these are seldom alike in two
different kinds of insects, so that there is here a wide field for the
student. The dissection, moreover, is very easy, as they may be cut
from the body with a sharp knife or scissors, and mounted in balsam or
fluid. Many of the larvæ afford good specimens, as do also some of the
common Coleopterous insects.
TONGUES, OR PALATES, OF MOLLUSCS.--Of the nature of these, Dr.
Carpenter gives the following description:--“The organ which is
commonly known under this designation is one of a very singular nature;
and we should be altogether wrong in conceiving of it as having any
likeness to that on which our ordinary ideas of such an organ are
founded. For, instead of being a projecting body, lying in the cavity
of the mouth, it is a tube that passes backwards and downwards beneath
the mouth, its higher end being closed, whilst in front it opens
obliquely upon the floor of the mouth, being, as it were, slit up and
spread out so as to form a nearly flat surface. On the interior of
the tube, as well as on the flat expansion of it, we find numerous
transverse rows of minute teeth, which are set upon flattened plates;
each principal tooth sometimes having a basal plate of its own, whilst
in other instances one plate carries several teeth.” These palates, or
tongues, differ much amongst the Gasteropods in form and size, some
of them being comparatively of an immense length. Many are amongst
the most beautiful objects when examined with polarized light. They
must, however, be procured by dissection, which is usually performed
as follows:--The animal is placed on the cork in the dissecting-trough
before mentioned, and the head and forepart cut open, spread out,
and firmly pinned down. With the aid of fine scissors or knife, the
tongue must be then detached from its fastenings, and placed in water
for a day or two, when all foreign matter may with a little care be
removed. In what way it should be mounted will depend on the purpose
for which it is intended. If for examination as an ordinary object, it
may be laid upon the slide and allowed to dry, which arrangement will
show the teeth very well. If we wish to see it as it is naturally, it
must be mounted in a cell with Goadby’s fluid; but if it is wanted as
a polarizing object, it must be floated upon a slide, allowed to dry
thoroughly, and then Canada balsam added in the usual manner.
In the stomach, also, of some of these molluscs teeth are found, which
are very interesting objects to examine, and must be dissected out in
the same manner as the “tongues.”
Since writing the above. Dr. Alcock (whose very beautiful specimens
prove him to be a great authority in this branch) has published some
of his experience in the second volume of the third series of “Memoirs
of the Literary and Philosophical Society of Manchester.” By his
permission I make the following extract:--
Public-domain text, read in full here on John Shaqi.
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