This Countess of Chinchon was a daughter of the noble house of Osorio, whose founder was created Marquis of Astorga by Henry IV., King of Castille. The eighth marquis, who died at Astorga in 1613, had a daughter by his wife Dona Blanca Manrique y Aragon, named Ana,[8] born in 1576; and the ruins of the palace in the curious old town of Astorga, in which she passed her childhood, are still standing.[9] At the early age of sixteen she was married to Don Luis de Velasco, Marquis of Salinas, who was about to assume the important office of viceroy of Mexico. She probably accompanied her husband to Mexico, and afterwards to Lima, as he was viceroy of Peru from 1596 to 1604. In the latter year he resumed his former office in Mexico, and, on his return to Spain, he became President of the Council of the Indies from 1611 to 1617.[10] The lady Ana had thus been a great traveller, when, in the latter year, she found herself a widow. In 1621 she was married, in the city of Madrid, to her second husband the fourth Count of Chinchon, who was descended from a long line of proud and valiant Catalonian ancestors. One of his forefathers, Don Andres de Cabrera, who was created Marquis of Moya in 1480, married Beatriz de Bobadilla, so well known in history as the faithful attendant and confidential friend of Queen Isabella the Catholic. The Emperor Charles V., remembering the services and ancient dignity of the illustrious families of Cabrera and Bobadilla, created the second son of the Marquis of Moya, by Beatriz de Bobadilla, Count of his town of Chinchon, in the kingdom of Toledo, in 1517.[11] The third Count was one of the over-worked ministers of that most indefatigable of "red-tapists" Philip II.; and his son became the husband of the widow Ana, who accompanied him to Lima on his appointment as viceroy of Peru in 1629. Thus, for the second time, this lady entered the City of the Kings as Vice-Queen. While the Countess Ana was suffering from fever in 1638, in her sixty-third year, the Corregidor of Loxa, Don Juan Lopez de Canizares, sent a parcel of powdered quinquina bark to her physician, Juan de Vega, who was also captain of the armoury, assuring him that it was a sovereign and never-failing remedy for "tertiana." It was administered to the Countess and effected a complete cure; and Mr. Howard is of opinion that the particular plant which had this honour, and which, therefore, yields the true and original Peruvian bark, is the _Chahuarguera_ variety of the _C. Condaminea_.[12] This kind contains a large percentage of _chinchonidine_, an alkaloid, the great importance of which is only now just beginning to be recognised, so that it is to _chinchonidine_, and not to _quinine_, that the Countess's cure is due.[13]