Common objects of the microscopeWood, J. G. (John George)
Science
Common objects of the microscope
Wood, J. G. (John George)
Microscopes; Microscopy
Many plants and animals require to be dissected to a certain extent
before the details of their structure can be made out, and for this
purpose the naked eye alone will rarely serve. The ordinary pocket
magnifier, however, if mounted as described in a preceding chapter,
will greatly facilitate matters, and the light may be focused upon the
object by means of the bull’s-eye condenser, as shown in Fig. 11. In
the figure the latter is represented as used in conjunction with the
lamp, but daylight is preferable if it be available, the strain upon
the eyes being very much less than with artificial light. Two blocks
of wood, about four inches high, will form convenient rests for the
hands, a plate of glass being placed upon the blocks to support the
dish, and a mirror being put in the interspace at an angle of 45° or
so if required. A piece of black paper may be laid upon the mirror if
reflected light alone is to be used.
As all delicate structures are dissected under fluid, a shallow dish
is required. For this purpose nothing is better than one of the dishes
used for developing photographic negatives. The bottom of the dish is
occupied by a flat cork, to which a piece of flat lead is attached
below, and the object having been pinned on to the cork in the required
position, the fluid is carefully run in. This fluid will naturally vary
according to the results desired to be obtained, but it must not be
plain water, which so alters all cellular structures as to practically
make them unrecognisable under the microscope. Nothing could be better
than a 5 per cent. solution of formalin, were it not for the somewhat
irritating odour of this fluid, since it at once fixes the cells and
preserves the figure. For many purposes a solution of salt, in the
proportion of a saltspoonful of the latter to a pint of water, will
answer well for short dissections. For more prolonged ones, a mixture
of spirit-and-water, one part of the former to two of the latter,
answers well, especially for vegetable structures. When the dilution
is first made, the fluid becomes milky, unless pure spirit be used,
but with a little trouble the Revenue authorities may be induced to
give permission for the use of pure methylated spirit, which answers
every purpose. The trouble then is that not less than five gallons can
be purchased, an _embarras de richesses_ for the average microscopist,
but, after all, the spirit is extremely cheap, and does not deteriorate
by keeping.
When the dissection in either of these media is completed, spirit
should be gradually added to bring the strength up to 50 per cent., in
which the preparation may remain for a day or two, after which it is
gradually brought into pure spirit, or into water again, according to
the medium in which it is to be mounted.
[Illustration: Fig. 12.]
Public-domain text, read in full here on John Shaqi.
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