In 1902, Preisz at Budapest isolated the same bacillus from two cases of contagious abortion in cows. He confirmed the findings of Bang in respect to the oxygen requirements of the organism, and was able to obtain cultures by a variety of methods on ordinary media. Apparently his cultures were less vigorous than those of Bang, for they soon died out, their resistance to germicides was slight, and all his inoculations into animals, including two pregnant cows, two pregnant guinea pigs, and one pregnant rabbit, as well as a number of other small animals, were without positive result. Preisz named the organism "_Corynebacterium abortus endemici (s. infectiosi)_." In 1908, Nowak at the University of Krakau in Austria made a very important contribution to the study of this disease. He found the culture method of Bang and Stribolt very useful for the identification of the organism when obtainable in pure culture, and when the contaminating bacteria were few in number. When other bacteria were numerous, as is frequently the case in material obtained for examination, he found this method difficult. The pyrogallol method of Preisz also proved to be unreliable in his hands. Eventually he devised a method of plate culture which proved to be very useful. Ordinary agar was melted and cooled to 50° C. then mixed with about one fourth its volume of naturally sterile blood serum, and poured into sterile Petri dishes where it was allowed to solidify. The piece of placenta or other material to be examined was then streaked over several of these plates in succession, and the plates were incubated for 24 hours at 37° C., to allow contaminating bacteria to develop. The plates were next placed in a glass jar together with a culture of _Bacillus subtilis_, one square centimeter of culture surface of the latter organism being provided for each 15 cc. capacity of the jar. The jar was sealed and placed at 37° C. for three days, at the end of which time excellent surface colonies of Bang's bacillus were obtained. By the application of this method Nowak has succeeded in isolating the bacillus from the blood and intestinal contents of a number of fetuses, and from uterine discharge, when other methods failed. He has also observed that one could gradually decrease the amount of culture surface of _B. subtilis_ employed in succeeding cultures and eventually get the bacillus of Bang to grow in the presence of atmospheric air, altho the cultures were relatively poor ones. Nowak also confirmed Bang by obtaining cultures in an atmosphere of nearly pure oxygen, as well as in ordinary air under a pressure of three atmospheres. His cultures were evidently vigorous for some of them were successfully transplanted after two years. Nowak used ordinary agar as a medium with considerable success, and found glucose agar to be almost as favorable to the growth of the bacillus. For the detection of the germ in pathological material, however, these media proved to be inferior to