On adipocire, and its formationWetherill, Charles Mayer
Science
On adipocire, and its formation
Wetherill, Charles Mayer
Adipocere; Fatty acids; Postmortem changes
A few experiments were now made with the alcoholic liquid 6^o. A
concentrated alcoholic solution of acetate of magnesia added to this
liquid, produced no precipitate, but micaceous crystalline scales fell
on adding acetic acid, and upon adding more acetic acid, and heating,
besides these crystals, an oil floated on the surface, which solidified
on cooling, and which behaved like a fat, and gave the melting point of
palmitic acid, viz.: 62° (solidifies gradually from 47° to 39°.) The
crystals gave a small quantity of ash when burned on platina foil, and
on being decomposed by hydrochloric acid, gave a fat with the melting
point of stearic acid 72°-73°, and solidifying at 60°-55°. The mother
liquid contained too little fat to experiment upon. To another portion
of the liquid 6^o, alcoholic acetate of magnesia was added without
addition of acetic acid, and the solution evaporated in a retort. The
first crystals which appeared contained a fat which fused at 65°-68.5°,
and solidified at 62°-58°.
The solid crystalline fat No. 6 which was removed from the liquid 6^o,
and which was the most highly purified result from the crystallization
of this specimen of adipocire, was now examined more particularly;
an alcoholic solution was made upon which to try the different
experiments. Fifteen grammes of the fat required 300 of alcohol of
93 per cent. to keep it in solution; but before having added so
much alcohol, on standing for a short time 0.656 grammes of pearly
crystalline scales fell, which had a melting point of 62.5°, and
solidified at 55.5°. The fat of the liquid after these crystals had
fallen, when precipitated by water, melted at 58°-61°, and solidified
at 55.5°: these crystals, recrystallized from alcohol, melted at 62.5°,
and solidified at 58°-57°; these were dissolved a third time, in
twenty times their weight of 93 per cent. alcohol, which deposited, on
standing, less than a milligramme of tufted crystals of the form of
palmitic acid, of which it had the melting point 62°: more alcohol was
added to the solution, and it was divided by fractional precipitation
with acetate of magnesia and the addition of a little ammonia with
heat, into two portions, weighing 0.256 and 0.164 grammes, and they had
the same melting point. This fat appears, therefore, to be palmitic
acid, one of the acids into which Heintz divided margaric acid. The
crystals deposited from alcohol do not at all resemble those of
margaric acid, but under the microscope are lamellar. These two fats
were converted respectively, by an excess of nitrate of silver, into
silver salts, 0·24725 gave 0·074 Ag. = 29·93 per cent. and 0·14275 gave
0·04175 Ag. = 29·25 per cent., which corresponds to the percentage of
silver in the palmitate of this base.
C_{32} 192.00 By calculation. Mean of two Exper.
H_{31} 31.00
O_{4} 32.00
Ag. 107.97 29.7 29.5
——————
362.97
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